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Literature summary extracted from

  • Delvaux, D.; Kerff, F.; Murty, M.R.; Lakaye, B.; Czerniecki, J.; Kohn, G.; Wins, P.; Herman, R.; Gabelica, V.; Heuze, F.; Tordoir, X.; Maree, R.; Matagne, A.; Charlier, P.; De Pauw, E.; Bettendorff, L.
    Structural determinants of specificity and catalytic mechanism in mammalian 25-kDa thiamine triphosphatase (2013), Biochim. Biophys. Acta, 1830, 4513-4523.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.28
-
 Homo sapiens
3.6.1.28
-
 Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.28 complex with tripolyphosphate, to 2.3 A resolution and docking solution of substrate thiamine triphosphate. The thiazole ring of the thiamine forms a stacking interaction with Trp53, and the aminopyrimidine ring lies in a pocket defined by His76, Pro191 and Ile195. The triphosphate moiety occupies a similar position as in the enzyme-tripolyphosphate complex, with interactions involving the side chains of Lys11, Arg55, Arg57, Lys65, Arg125 and Lys193 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.28 D147A about 30% of wild-type catalytic efficiency Mus musculus
3.6.1.28 D37A about 0.5% of wild-type catalytic efficiency Mus musculus
3.6.1.28 E81A about 30% of wild-type catalytic efficiency Mus musculus
3.6.1.28 K11A about 15% of wild-type catalytic efficiency Mus musculus
3.6.1.28 K193A about 60% of wild-type catalytic efficiency Mus musculus
3.6.1.28 K65A more than 1000fold decrease in kcat value Mus musculus
3.6.1.28 W53A 2.5fold increase in catalytic efficiency Mus musculus
3.6.1.28 Y39A about 30% of wild-type catalytic efficiency Mus musculus
3.6.1.28 Y79A about 5% of wild-type catalytic efficiency Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.28 0.008
-
 thiamine triphosphate wild-type, pH 8.5, 37°C Mus musculus
3.6.1.28 0.009
-
 thiamine triphosphate mutant K65A, pH 8.5, 37°C Mus musculus
3.6.1.28 0.012
-
 thiamine triphosphate mutant K11A, pH 8.5, 37°C Mus musculus
3.6.1.28 0.013
-
 thiamine triphosphate mutant E81A, pH 8.5, 37°C Mus musculus
3.6.1.28 0.042
-
 thiamine triphosphate mutant Y79A, pH 8.5, 37°C Mus musculus
3.6.1.28 0.2
-
 thiamine triphosphate mutant D37A, pH 8.5, 37°C Mus musculus
3.6.1.28 0.55
-
 thiamine triphosphate mutant W53A, pH 8.5, 37°C Mus musculus
3.6.1.28 0.62
-
 thiamine triphosphate mutant Y39A, pH 8.5, 37°C Mus musculus
3.6.1.28 0.74
-
 thiamine triphosphate mutant K193A, pH 8.5, 37°C Mus musculus
3.6.1.28 2.8
-
 thiamine triphosphate mutant D147A, pH 8.5, 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.28 Mg2+ required for catalytic activity, not required for substrate binding Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.28 Homo sapiens Q9BU02
-
-
3.6.1.28 Mus musculus Q8JZL3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.28 thiamine triphosphate + H2O
-
 Mus musculus thiamine diphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.28 Thtpa
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.28 0.019
-
 thiamine triphosphate mutant K65A, pH 8.5, 37°C Mus musculus
3.6.1.28 0.64
-
 thiamine triphosphate mutant Y39A, pH 8.5, 37°C Mus musculus
3.6.1.28 1.5
-
 thiamine triphosphate mutant K193A, pH 8.5, 37°C Mus musculus
3.6.1.28 2.6
-
 thiamine triphosphate mutant D147A, pH 8.5, 37°C Mus musculus
3.6.1.28 2.8
-
 thiamine triphosphate mutant D37A, pH 8.5, 37°C Mus musculus
3.6.1.28 4.4
-
 thiamine triphosphate mutant W53A, pH 8.5, 37°C Mus musculus
3.6.1.28 6
-
 thiamine triphosphate mutant K11A, pH 8.5, 37°C Mus musculus
3.6.1.28 6
-
 thiamine triphosphate mutant Y79A, pH 8.5, 37°C Mus musculus
3.6.1.28 14.1
-
 thiamine triphosphate mutant E81A, pH 8.5, 37°C Mus musculus
3.6.1.28 23
-
 thiamine triphosphate wild-type, pH 8.5, 37°C Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.28 14
-
 thiamine triphosphate mutant D37A, pH 8.5, 37°C Mus musculus
3.6.1.28 140
-
 thiamine triphosphate mutant Y79A, pH 8.5, 37°C Mus musculus
3.6.1.28 500
-
 thiamine triphosphate mutant K11A, pH 8.5, 37°C Mus musculus
3.6.1.28 1000
-
 thiamine triphosphate mutant D147A, pH 8.5, 37°C Mus musculus
3.6.1.28 1000
-
 thiamine triphosphate mutant E81A, pH 8.5, 37°C Mus musculus
3.6.1.28 1000
-
 thiamine triphosphate mutant Y39A, pH 8.5, 37°C Mus musculus
3.6.1.28 2000
-
 thiamine triphosphate mutant K193A, pH 8.5, 37°C Mus musculus
3.6.1.28 2000
-
 thiamine triphosphate mutant K65A, pH 8.5, 37°C Mus musculus
3.6.1.28 3000
-
 thiamine triphosphate wild-type, pH 8.5, 37°C Mus musculus
3.6.1.28 8000
-
 thiamine triphosphate mutant W53A, pH 8.5, 37°C Mus musculus