BRENDA - Enzyme Database show

Structure of an As(III) S-adenosylmethionine methyltransferase: insights into the mechanism of arsenic biotransformation

Ajees, A.A.; Marapakala, K.; Packianathan, C.; Sankaran, B.; Rosen, B.P.; Biochemistry 51, 5476-5485 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21(DE3) cells
Cyanidioschyzon sp.
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.1.1.137
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3000, 0.1 M Tris-HCl, pH 7.0, containing 0.2 M calcium acetate
Cyanidioschyzon sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
44980
-
x * 44980, calculated from amino acid sequence
Cyanidioschyzon sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Cyanidioschyzon sp.
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
Cyanidioschyzon sp. 5508
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Cyanidioschyzon sp.
-
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Cyanidioschyzon sp. 5508
-
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Cyanidioschyzon sp.
C0JV69
-
-
2.1.1.137
Cyanidioschyzon sp. 5508
C0JV69
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.137
-
Cyanidioschyzon sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733333
Cyanidioschyzon sp.
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733333
Cyanidioschyzon sp. 5508
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733333
Cyanidioschyzon sp.
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733333
Cyanidioschyzon sp. 5508
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 44980, calculated from amino acid sequence
Cyanidioschyzon sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21(DE3) cells
Cyanidioschyzon sp.
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.1.1.137
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3000, 0.1 M Tris-HCl, pH 7.0, containing 0.2 M calcium acetate
Cyanidioschyzon sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
44980
-
x * 44980, calculated from amino acid sequence
Cyanidioschyzon sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Cyanidioschyzon sp.
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
Cyanidioschyzon sp. 5508
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Cyanidioschyzon sp.
-
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Cyanidioschyzon sp. 5508
-
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
-
Cyanidioschyzon sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733333
Cyanidioschyzon sp.
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733333
Cyanidioschyzon sp. 5508
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733333
Cyanidioschyzon sp.
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733333
Cyanidioschyzon sp. 5508
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 44980, calculated from amino acid sequence
Cyanidioschyzon sp.