BRENDA - Enzyme Database show

Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahymena pyriformis

Ye, J.; Chang, Y.; Yan, Y.; Xiong, J.; Xue, X.M.; Yuan, D.; Sun, G.X.; Zhu, Y.G.; Miao, W.; Aquat. Toxicol. 149, 50-57 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli DH5alpha and AW3110 cells
Tetrahymena pyriformis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
30000
-
x * 30000, His-tagged enzyme, SDS-PAGE
Tetrahymena pyriformis
2.1.1.137
35400
-
x * 35400, His-tagged enzyme, calculated from amino acid sequence
Tetrahymena pyriformis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
additional information
Tetrahymena pyriformis
besides methylarsonate and dimethylarsinate, the enzyme also produces trimethylarsenite and dimethylarsine gases
?
-
-
-
2.1.1.137
S-adenosyl-L-methionine + arsenite
Tetrahymena pyriformis
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Tetrahymena pyriformis
-
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Tetrahymena pyriformis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.137
HisTrap column chromatography
Tetrahymena pyriformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
additional information
besides methylarsonate and dimethylarsinate, the enzyme also produces trimethylarsenite and dimethylarsine gases
733203
Tetrahymena pyriformis
?
-
-
-
-
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733203
Tetrahymena pyriformis
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733203
Tetrahymena pyriformis
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 30000, His-tagged enzyme, SDS-PAGE; x * 35400, His-tagged enzyme, calculated from amino acid sequence
Tetrahymena pyriformis
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.1.1.137
16
37
purified enzyme converts almost all arsenite to dimethylarsinate at temperatures of 16–37°C in pH 7.5 buffer, while at higher temperatures the rate of arsenite transformation is reduced
Tetrahymena pyriformis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
7.5
-
-
Tetrahymena pyriformis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli DH5alpha and AW3110 cells
Tetrahymena pyriformis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
30000
-
x * 30000, His-tagged enzyme, SDS-PAGE
Tetrahymena pyriformis
2.1.1.137
35400
-
x * 35400, His-tagged enzyme, calculated from amino acid sequence
Tetrahymena pyriformis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
additional information
Tetrahymena pyriformis
besides methylarsonate and dimethylarsinate, the enzyme also produces trimethylarsenite and dimethylarsine gases
?
-
-
-
2.1.1.137
S-adenosyl-L-methionine + arsenite
Tetrahymena pyriformis
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Tetrahymena pyriformis
-
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
HisTrap column chromatography
Tetrahymena pyriformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
additional information
besides methylarsonate and dimethylarsinate, the enzyme also produces trimethylarsenite and dimethylarsine gases
733203
Tetrahymena pyriformis
?
-
-
-
-
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733203
Tetrahymena pyriformis
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733203
Tetrahymena pyriformis
S-adenosyl-L-homocysteine + dimethylarsinate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 30000, His-tagged enzyme, SDS-PAGE; x * 35400, His-tagged enzyme, calculated from amino acid sequence
Tetrahymena pyriformis
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.1.1.137
16
37
purified enzyme converts almost all arsenite to dimethylarsinate at temperatures of 16–37°C in pH 7.5 buffer, while at higher temperatures the rate of arsenite transformation is reduced
Tetrahymena pyriformis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
7.5
-
-
Tetrahymena pyriformis