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Literature summary extracted from

  • Ye, J.; Chang, Y.; Yan, Y.; Xiong, J.; Xue, X.M.; Yuan, D.; Sun, G.X.; Zhu, Y.G.; Miao, W.
    Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahymena pyriformis (2014), Aquat. Toxicol., 149, 50-57.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.137 expressed in Escherichia coli DH5alpha and AW3110 cells Tetrahymena pyriformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.137 30000
-
 x * 30000, His-tagged enzyme, SDS-PAGE Tetrahymena pyriformis
2.1.1.137 35400
-
 x * 35400, His-tagged enzyme, calculated from amino acid sequence Tetrahymena pyriformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.137 additional information Tetrahymena pyriformis besides methylarsonate and dimethylarsinate, the enzyme also produces trimethylarsenite and dimethylarsine gases ?
-
 ?
2.1.1.137 S-adenosyl-L-methionine + arsenite Tetrahymena pyriformis
-
 S-adenosyl-L-homocysteine + methylarsonate
-
 ?
2.1.1.137 S-adenosyl-L-methionine + methylarsonite Tetrahymena pyriformis
-
 S-adenosyl-L-homocysteine + dimethylarsinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.137 Tetrahymena pyriformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.137 HisTrap column chromatography Tetrahymena pyriformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.137 additional information besides methylarsonate and dimethylarsinate, the enzyme also produces trimethylarsenite and dimethylarsine gases Tetrahymena pyriformis ?
-
 ?
2.1.1.137 S-adenosyl-L-methionine + arsenite
-
 Tetrahymena pyriformis S-adenosyl-L-homocysteine + methylarsonate
-
 ?
2.1.1.137 S-adenosyl-L-methionine + methylarsonite
-
 Tetrahymena pyriformis S-adenosyl-L-homocysteine + dimethylarsinate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.137 ? x * 30000, His-tagged enzyme, SDS-PAGE Tetrahymena pyriformis
2.1.1.137 ? x * 35400, His-tagged enzyme, calculated from amino acid sequence Tetrahymena pyriformis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.137 arsenite methyltransferase
-
 Tetrahymena pyriformis
2.1.1.137 ArsM
-
 Tetrahymena pyriformis
2.1.1.137 As(III) methyltransferase
-
 Tetrahymena pyriformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.137 16 37 purified enzyme converts almost all arsenite to dimethylarsinate at temperatures of 16–37°C in pH 7.5 buffer, while at higher temperatures the rate of arsenite transformation is reduced Tetrahymena pyriformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.137 7.5
-
-
 Tetrahymena pyriformis