BRENDA - Enzyme Database show

Arsenic methylation and volatilization by arsenite S-adenosylmethionine methyltransferase in Pseudomonas alcaligenes NBRC14159

Zhang, J.; Cao, T.; Tang, Z.; Shen, Q.; Rosen, B.P.; Zhao, F.J.; Appl. Environ. Microbiol. 81, 2852-2860 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas alcaligenes
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
38000
-
x * 38000, calculated from amino acid sequence
Pseudomonas alcaligenes
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Pseudomonas alcaligenes
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
Pseudomonas alcaligenes NBRC 14159
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Pseudomonas alcaligenes
-
S-adenosyl-L-homocysteine + dimethylarsinate
the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Pseudomonas alcaligenes NBRC 14159
-
S-adenosyl-L-homocysteine + dimethylarsinate
the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Pseudomonas alcaligenes
U2ZU49
-
-
2.1.1.137
Pseudomonas alcaligenes NBRC 14159
U2ZU49
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.137
Ni-NTA column chromatography
Pseudomonas alcaligenes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733175
Pseudomonas alcaligenes
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733175
Pseudomonas alcaligenes NBRC 14159
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733175
Pseudomonas alcaligenes
S-adenosyl-L-homocysteine + dimethylarsinate
the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733175
Pseudomonas alcaligenes NBRC 14159
S-adenosyl-L-homocysteine + dimethylarsinate
the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 38000, calculated from amino acid sequence
Pseudomonas alcaligenes
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.1.1.137
37
-
-
Pseudomonas alcaligenes
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
7.5
-
-
Pseudomonas alcaligenes
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas alcaligenes
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
38000
-
x * 38000, calculated from amino acid sequence
Pseudomonas alcaligenes
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Pseudomonas alcaligenes
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
Pseudomonas alcaligenes NBRC 14159
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Pseudomonas alcaligenes
-
S-adenosyl-L-homocysteine + dimethylarsinate
the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
Pseudomonas alcaligenes NBRC 14159
-
S-adenosyl-L-homocysteine + dimethylarsinate
the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
Ni-NTA column chromatography
Pseudomonas alcaligenes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733175
Pseudomonas alcaligenes
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733175
Pseudomonas alcaligenes NBRC 14159
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733175
Pseudomonas alcaligenes
S-adenosyl-L-homocysteine + dimethylarsinate
the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products
-
-
?
2.1.1.137
S-adenosyl-L-methionine + methylarsonite
-
733175
Pseudomonas alcaligenes NBRC 14159
S-adenosyl-L-homocysteine + dimethylarsinate
the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 38000, calculated from amino acid sequence
Pseudomonas alcaligenes
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.1.1.137
37
-
-
Pseudomonas alcaligenes
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
7.5
-
-
Pseudomonas alcaligenes
Expression
EC Number
Organism
Commentary
Expression
2.1.1.137
Pseudomonas alcaligenes
the enzyme is expressed in the absence of As(3+), and the expression is further enhanced after 6 h by 0.04 mM As(3+) exposure
up
General Information
EC Number
General Information
Commentary
Organism
2.1.1.137
malfunction
a gene disruption mutant loses arsenite methylation ability and becomes more sensitive to arsenite
Pseudomonas alcaligenes
2.1.1.137
physiological function
the enzyme plays a role in arsenite methylation and detoxification of As3+
Pseudomonas alcaligenes
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.137
malfunction
a gene disruption mutant loses arsenite methylation ability and becomes more sensitive to arsenite
Pseudomonas alcaligenes
2.1.1.137
physiological function
the enzyme plays a role in arsenite methylation and detoxification of As3+
Pseudomonas alcaligenes
Expression (protein specific)
EC Number
Organism
Commentary
Expression
2.1.1.137
Pseudomonas alcaligenes
the enzyme is expressed in the absence of As(3+), and the expression is further enhanced after 6 h by 0.04 mM As(3+) exposure
up