Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, J.; Cao, T.; Tang, Z.; Shen, Q.; Rosen, B.P.; Zhao, F.J.
    Arsenic methylation and volatilization by arsenite S-adenosylmethionine methyltransferase in Pseudomonas alcaligenes NBRC14159 (2015), Appl. Environ. Microbiol., 81, 2852-2860.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.137 expressed in Escherichia coli BL21(DE3) cells Pseudomonas alcaligenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.137 38000
-
 x * 38000, calculated from amino acid sequence Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite Pseudomonas alcaligenes
-
 S-adenosyl-L-homocysteine + methylarsonate
-
 ?
2.1.1.137 S-adenosyl-L-methionine + arsenite Pseudomonas alcaligenes NBRC 14159
-
 S-adenosyl-L-homocysteine + methylarsonate
-
 ?
2.1.1.137 S-adenosyl-L-methionine + methylarsonite Pseudomonas alcaligenes
-
 S-adenosyl-L-homocysteine + dimethylarsinate the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products ?
2.1.1.137 S-adenosyl-L-methionine + methylarsonite Pseudomonas alcaligenes NBRC 14159
-
 S-adenosyl-L-homocysteine + dimethylarsinate the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.137 Pseudomonas alcaligenes U2ZU49
-
-
2.1.1.137 Pseudomonas alcaligenes NBRC 14159 U2ZU49
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.137 Ni-NTA column chromatography Pseudomonas alcaligenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite
-
 Pseudomonas alcaligenes S-adenosyl-L-homocysteine + methylarsonate
-
 ?
2.1.1.137 S-adenosyl-L-methionine + arsenite
-
 Pseudomonas alcaligenes NBRC 14159 S-adenosyl-L-homocysteine + methylarsonate
-
 ?
2.1.1.137 S-adenosyl-L-methionine + methylarsonite
-
 Pseudomonas alcaligenes S-adenosyl-L-homocysteine + dimethylarsinate the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products ?
2.1.1.137 S-adenosyl-L-methionine + methylarsonite
-
 Pseudomonas alcaligenes NBRC 14159 S-adenosyl-L-homocysteine + dimethylarsinate the enzyme also produces also produces dimethylarsine and trimethylarsine gases as by-products ?

Subunits

EC Number Subunits Comment Organism
2.1.1.137 ? x * 38000, calculated from amino acid sequence Pseudomonas alcaligenes

Synonyms

EC Number Synonyms Comment Organism
2.1.1.137 arsenite S-adenosylmethionine methyltransferase
-
 Pseudomonas alcaligenes
2.1.1.137 ArsM
-
 Pseudomonas alcaligenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.137 37
-
-
 Pseudomonas alcaligenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.137 7.5
-
-
 Pseudomonas alcaligenes

Expression

EC Number Organism Comment Expression
2.1.1.137 Pseudomonas alcaligenes the enzyme is expressed in the absence of As(3+), and the expression is further enhanced after 6 h by 0.04 mM As(3+) exposure up

General Information

EC Number General Information Comment Organism
2.1.1.137 malfunction a gene disruption mutant loses arsenite methylation ability and becomes more sensitive to arsenite Pseudomonas alcaligenes
2.1.1.137 physiological function the enzyme plays a role in arsenite methylation and detoxification of As3+ Pseudomonas alcaligenes