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Literature summary extracted from

  • Tavares, S.; Grotkjaer, T.; Obsen, T.; Haslam, R.; Napier, J.; Gunnarsson, N.
    Metabolic engineering of Saccharomyces cerevisiae for production of eicosapentaenoic acid, using a novel DELTA5-desaturase from Paramecium tetraurelia (2011), Appl. Environ. Microbiol., 77, 1854-1861.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.30 expressed in Saccharoymces cerevisiae strain CEN.PK 113-5D Leishmania major
1.14.19.30 expressed in Saccharoymces cerevisiae strain CEN.PK 113-5D Paramecium tetraurelia
1.14.19.30 expressed in Saccharoymces cerevisiae strain CEN.PK 113-5D Mortierella alpina
1.14.19.30 expressed in Saccharoymces cerevisiae strain CEN.PK 113-5D Ostreococcus tauri
1.14.19.30 expressed in Saccharoymces cerevisiae strain CEN.PK 113-5D Ostreococcus sp. 'lucimarinus'
1.14.19.44 expressed in Saccharomyces cerevisiae strain CEN.PK 113-5D Paramecium tetraurelia
1.14.19.44 expressed in Saccharomyces cerevisiae strain CEN.PK 113-5D Ostreococcus tauri
1.14.19.44 expressed in Saccharomyces cerevisiae strain CEN.PK 113-5D Ostreococcus sp. 'lucimarinus'
1.14.19.44 expressed in Saccharomyces cerevisiae strain CEN.PK 113-5D Leishmania major
1.14.19.44 expressed in Saccharomyces cerevisiae strain CEN.PK 113-5D Mortierella alpina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Leishmania major
-
 (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Paramecium tetraurelia
-
 (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Mortierella alpina
-
 (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Ostreococcus tauri
-
 (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Ostreococcus sp. 'lucimarinus'
-
 (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Leishmania major
-
 (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Paramecium tetraurelia
-
 (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Mortierella alpina
-
 (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Ostreococcus tauri
-
 (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Ostreococcus sp. 'lucimarinus'
-
 (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.30 Leishmania major
-
-
-
1.14.19.30 Mortierella alpina
-
-
-
1.14.19.30 Ostreococcus sp. 'lucimarinus'
-
-
-
1.14.19.30 Ostreococcus tauri
-
-
-
1.14.19.30 Paramecium tetraurelia
-
-
-
1.14.19.44 Leishmania major Q4QIH7
-
-
1.14.19.44 Mortierella alpina D5KSD5
-
-
1.14.19.44 Mortierella alpina CBS 608.70 D5KSD5
-
-
1.14.19.44 Ostreococcus sp. 'lucimarinus'
-
-
-
1.14.19.44 Ostreococcus tauri
-
-
-
1.14.19.44 Paramecium tetraurelia
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Leishmania major (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Paramecium tetraurelia (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus tauri (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus sp. 'lucimarinus' (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Leishmania major (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Paramecium tetraurelia (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus tauri (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.30 (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus sp. 'lucimarinus' (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Paramecium tetraurelia arachidonoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus tauri arachidonoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus sp. 'lucimarinus' arachidonoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Leishmania major arachidonoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina arachidonoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina CBS 608.70 arachidonoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Paramecium tetraurelia arachidonic acid-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus tauri arachidonic acid-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus sp. 'lucimarinus' arachidonic acid-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina arachidonic acid-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 dihomo-gamma-linolenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina CBS 608.70 arachidonic acid-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Paramecium tetraurelia eicosapentaenoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus tauri eicosapentaenoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus sp. 'lucimarinus' eicosapentaenoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Leishmania major eicosapentaenoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina eicosapentaenoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-CoA + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina CBS 608.70 eicosapentaenoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Paramecium tetraurelia eicosapentaenoyl-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus tauri eicosapentaenoyl-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Ostreococcus sp. 'lucimarinus' eicosapentaenoyl-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina eicosapentaenoyl-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 eicosatetraenoyl-phospholipid + ferrocytochrome b5 + O2 + H+
-
 Mortierella alpina CBS 608.70 eicosapentaenoyl-phospholipid + ferricytochrome b5 + H2O
-
 ?
1.14.19.44 additional information the enzyme does not accept phospholipid-bound substrates Leishmania major ?
-
 ?
1.14.19.44 additional information the enzyme is promiscuous toward the acyl carrier substrate but prefers phospholipid-bound substrates Ostreococcus tauri ?
-
 ?
1.14.19.44 additional information the enzyme is promiscuous toward the acyl carrier substrate but prefers phospholipid-bound substrates Ostreococcus sp. 'lucimarinus' ?
-
 ?
1.14.19.44 additional information the enzyme is promiscuous toward the acyl carrier substrate but prefers phospholipid-bound substrates Mortierella alpina ?
-
 ?
1.14.19.44 additional information the enzyme promiscuous toward the acyl carrier substrate but prefers phospholipid-bound substrates Paramecium tetraurelia ?
-
 ?
1.14.19.44 additional information the enzyme is promiscuous toward the acyl carrier substrate but prefers phospholipid-bound substrates Mortierella alpina CBS 608.70 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.30 D5D
-
 Leishmania major
1.14.19.30 D5D
-
 Paramecium tetraurelia
1.14.19.30 D5D
-
 Mortierella alpina
1.14.19.30 D5D
-
 Ostreococcus tauri
1.14.19.30 D5D
-
 Ostreococcus sp. 'lucimarinus'
1.14.19.30 DELTA5 desaturase
-
 Leishmania major
1.14.19.30 DELTA5 desaturase
-
 Paramecium tetraurelia
1.14.19.30 DELTA5 desaturase
-
 Mortierella alpina
1.14.19.30 DELTA5 desaturase
-
 Ostreococcus tauri
1.14.19.30 DELTA5 desaturase
-
 Ostreococcus sp. 'lucimarinus'
1.14.19.44 D5D
-
 Paramecium tetraurelia
1.14.19.44 D5D
-
 Ostreococcus tauri
1.14.19.44 D5D
-
 Ostreococcus sp. 'lucimarinus'
1.14.19.44 D5D
-
 Leishmania major
1.14.19.44 D5D
-
 Mortierella alpina
1.14.19.44 DELTA5-desaturase
-
 Paramecium tetraurelia
1.14.19.44 DELTA5-desaturase
-
 Ostreococcus tauri
1.14.19.44 DELTA5-desaturase
-
 Ostreococcus sp. 'lucimarinus'
1.14.19.44 DELTA5-desaturase
-
 Leishmania major
1.14.19.44 DELTA5-desaturase
-
 Mortierella alpina

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.30 cytochrome b5
-
 Leishmania major
1.14.19.30 cytochrome b5
-
 Paramecium tetraurelia
1.14.19.30 cytochrome b5
-
 Mortierella alpina
1.14.19.30 cytochrome b5
-
 Ostreococcus tauri
1.14.19.30 cytochrome b5
-
 Ostreococcus sp. 'lucimarinus'
1.14.19.44 cytochrome b5
-
 Paramecium tetraurelia
1.14.19.44 cytochrome b5
-
 Ostreococcus tauri
1.14.19.44 cytochrome b5
-
 Ostreococcus sp. 'lucimarinus'
1.14.19.44 cytochrome b5
-
 Leishmania major
1.14.19.44 cytochrome b5
-
 Mortierella alpina