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Literature summary extracted from

  • Arthurs, C.E.; Lloyd, D.
    Kinetics, stereospecificity, and expression of the malolactic enzyme (1999), Appl. Environ. Microbiol., 65, 3360-3363.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.101 3.7
-
 L-malate pH 4.5, 25°C, activity of cell extract Secundilactobacillus collinoides
4.1.1.101 8
-
 DL-malate pH 4.5, 25°C, activity of cell extract Secundilactobacillus collinoides
4.1.1.101 8.3
-
 D-malate pH 4.5, 25°C, activity of cell extract Secundilactobacillus collinoides

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.101 Secundilactobacillus collinoides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.101 D-malate
-
 Secundilactobacillus collinoides D-lactate + CO2
-
 ?
4.1.1.101 DL-malate
-
 Secundilactobacillus collinoides DL-lactate + CO2
-
 ?
4.1.1.101 L-malate
-
 Secundilactobacillus collinoides L-lactate + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.101 4.9
-
-
 Secundilactobacillus collinoides

Expression

EC Number Organism Comment Expression
4.1.1.101 Secundilactobacillus collinoides enzyme is constitutively expressed. Addition of L-malic acid (37 mM) to the growth medium results in increased activity, addition of the D-isomer alone or the racemic mixture results in lower activities. Addition of the main sugars in apple juice (fructose, sucrose, and glucose) to the growth medium in the presence of malic acid represses production of the enzyme. In the absence of malic acid, addition of sugars to the growth medium somewhat increases the residual malolactic activity additional information