BRENDA - Enzyme Database

Novel aminoglycoside 2-phosphotransferase identified in a gram-negative pathogen

Toth, M.; Frase, H.; Antunes, N.T.; Vakulenko, S.B.; Antimicrob. Agents Chemother. 57, 452-457 (2013)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.190
lividomycin A
-
Campylobacter jejuni
2.7.1.190
neamine
-
Campylobacter jejuni
2.7.1.190
neomycin B
-
Campylobacter jejuni
2.7.1.190
paromomycin
-
Campylobacter jejuni
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.00055
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0007
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0008
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.001
-
tobramycin
above 0.001, pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0011
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0012
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0016
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0062
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.01
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.26
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.56
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
2
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.190
Campylobacter jejuni
Q4VR94
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.190
ATP + gentamicin
-
733113
Campylobacter jejuni
ADP + gentamicin 2''-phosphate
GTP is preferred over ATP
-
-
?
2.7.1.190
GTP + amikacin
-
733113
Campylobacter jejuni
GDP + amikacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + arbekacin
-
733113
Campylobacter jejuni
GDP + arbekacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + dibekacin
-
733113
Campylobacter jejuni
GDP + dibekacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + gentamicin C
-
733113
Campylobacter jejuni
GDP + gentamicin C 2''-phosphate
GTP is preferred over ATP
-
-
?
2.7.1.190
GTP + isepamicin
-
733113
Campylobacter jejuni
GDP + isepamicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + kanamycin A
-
733113
Campylobacter jejuni
GDP + kanamycin A 2''-phosphate
-
-
-
?
2.7.1.190
GTP + kanamycin B
-
733113
Campylobacter jejuni
GDP + kanamycin B 2''-phosphate
-
-
-
?
2.7.1.190
GTP + netilmicin
-
733113
Campylobacter jejuni
GDP + netilmicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + sisomicin
-
733113
Campylobacter jejuni
GDP + sisomicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + tobramycin
-
733113
Campylobacter jejuni
GDP + tobramycin 2''-phosphate
-
-
-
?
2.7.1.190
additional information
no substrates: 4,5-disubstituted antibiotics and the atypical aminoglycoside neamine
733113
Campylobacter jejuni
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.25
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.38
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.38
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.4
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.48
-
tobramycin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.79
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
1.7
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
4.3
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
6.5
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
8.3
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
17
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
18
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.190
0.00016
-
neamine
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.00073
-
neomycin B
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0085
-
paromomycin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.012
-
lividomycin A
pH 7.0, 22°C
Campylobacter jejuni
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.190
lividomycin A
-
Campylobacter jejuni
2.7.1.190
neamine
-
Campylobacter jejuni
2.7.1.190
neomycin B
-
Campylobacter jejuni
2.7.1.190
paromomycin
-
Campylobacter jejuni
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.190
0.00016
-
neamine
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.00073
-
neomycin B
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0085
-
paromomycin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.012
-
lividomycin A
pH 7.0, 22°C
Campylobacter jejuni
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.00055
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0007
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0008
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.001
-
tobramycin
above 0.001, pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0011
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0012
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0016
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.0062
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.01
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.26
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.56
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
2
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.190
ATP + gentamicin
-
733113
Campylobacter jejuni
ADP + gentamicin 2''-phosphate
GTP is preferred over ATP
-
-
?
2.7.1.190
GTP + amikacin
-
733113
Campylobacter jejuni
GDP + amikacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + arbekacin
-
733113
Campylobacter jejuni
GDP + arbekacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + dibekacin
-
733113
Campylobacter jejuni
GDP + dibekacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + gentamicin C
-
733113
Campylobacter jejuni
GDP + gentamicin C 2''-phosphate
GTP is preferred over ATP
-
-
?
2.7.1.190
GTP + isepamicin
-
733113
Campylobacter jejuni
GDP + isepamicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + kanamycin A
-
733113
Campylobacter jejuni
GDP + kanamycin A 2''-phosphate
-
-
-
?
2.7.1.190
GTP + kanamycin B
-
733113
Campylobacter jejuni
GDP + kanamycin B 2''-phosphate
-
-
-
?
2.7.1.190
GTP + netilmicin
-
733113
Campylobacter jejuni
GDP + netilmicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + sisomicin
-
733113
Campylobacter jejuni
GDP + sisomicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + tobramycin
-
733113
Campylobacter jejuni
GDP + tobramycin 2''-phosphate
-
-
-
?
2.7.1.190
additional information
no substrates: 4,5-disubstituted antibiotics and the atypical aminoglycoside neamine
733113
Campylobacter jejuni
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.25
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.38
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.38
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.4
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.48
-
tobramycin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.79
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
1.7
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
4.3
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
6.5
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
8.3
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
17
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
18
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
General Information
EC Number
General Information
Commentary
Organism
2.7.1.190
physiological function
expression in Escherichia coli confers resistance to the 4,6-disubstituted aminoglycosides kanamycin, tobramycin, dibekacin, gentamicin, and sisomicin, but not to arbekacin, amikacin, isepamicin, or netilmicin, but not to any of the 4,5-disubstituted antibiotics tested
Campylobacter jejuni
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.190
physiological function
expression in Escherichia coli confers resistance to the 4,6-disubstituted aminoglycosides kanamycin, tobramycin, dibekacin, gentamicin, and sisomicin, but not to arbekacin, amikacin, isepamicin, or netilmicin, but not to any of the 4,5-disubstituted antibiotics tested
Campylobacter jejuni
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.19
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.45
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
25
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
480
-
tobramycin
above 480, pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
510
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
660
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
690
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
690
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
1500
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
1700
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
11000
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
12000
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.19
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
0.45
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
25
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
480
-
tobramycin
above 480, pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
510
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
660
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
690
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
690
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
1500
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
1700
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
11000
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
2.7.1.190
12000
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni