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Literature summary extracted from

  • Toth, M.; Frase, H.; Antunes, N.T.; Vakulenko, S.B.
    Novel aminoglycoside 2-phosphotransferase identified in a gram-negative pathogen (2013), Antimicrob. Agents Chemother., 57, 452-457.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.190 lividomycin A
-
 Campylobacter jejuni
2.7.1.190 neamine
-
 Campylobacter jejuni
2.7.1.190 neomycin B
-
 Campylobacter jejuni
2.7.1.190 paromomycin
-
 Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.190 0.00055
-
 kanamycin B pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.0007
-
 gentamicin C pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.0008
-
 GTP pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.001
-
 tobramycin above 0.001, pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.0011
-
 kanamycin A pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.0012
-
 dibekacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.0016
-
 sisomicin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.0062
-
 arbekacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.01
-
 netilmicin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.26
-
 amikacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.56
-
 isepamicin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 2
-
 ATP pH 7.0, 22°C Campylobacter jejuni

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.190 Campylobacter jejuni Q4VR94
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.190 ATP + gentamicin
-
 Campylobacter jejuni ADP + gentamicin 2''-phosphate GTP is preferred over ATP ?
2.7.1.190 GTP + amikacin
-
 Campylobacter jejuni GDP + amikacin 2''-phosphate
-
 ?
2.7.1.190 GTP + arbekacin
-
 Campylobacter jejuni GDP + arbekacin 2''-phosphate
-
 ?
2.7.1.190 GTP + dibekacin
-
 Campylobacter jejuni GDP + dibekacin 2''-phosphate
-
 ?
2.7.1.190 GTP + gentamicin C
-
 Campylobacter jejuni GDP + gentamicin C 2''-phosphate GTP is preferred over ATP ?
2.7.1.190 GTP + isepamicin
-
 Campylobacter jejuni GDP + isepamicin 2''-phosphate
-
 ?
2.7.1.190 GTP + kanamycin A
-
 Campylobacter jejuni GDP + kanamycin A 2''-phosphate
-
 ?
2.7.1.190 GTP + kanamycin B
-
 Campylobacter jejuni GDP + kanamycin B 2''-phosphate
-
 ?
2.7.1.190 GTP + netilmicin
-
 Campylobacter jejuni GDP + netilmicin 2''-phosphate
-
 ?
2.7.1.190 GTP + sisomicin
-
 Campylobacter jejuni GDP + sisomicin 2''-phosphate
-
 ?
2.7.1.190 GTP + tobramycin
-
 Campylobacter jejuni GDP + tobramycin 2''-phosphate
-
 ?
2.7.1.190 additional information no substrates: 4,5-disubstituted antibiotics and the atypical aminoglycoside neamine Campylobacter jejuni ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.190 Aph(2'')-If
-
 Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.190 0.25
-
 isepamicin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.38
-
 ATP pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.38
-
 kanamycin B pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.4
-
 GTP pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.48
-
 tobramycin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.79
-
 dibekacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 1.7
-
 kanamycin A pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 4.3
-
 arbekacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 6.5
-
 amikacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 8.3
-
 gentamicin C pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 17
-
 sisomicin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 18
-
 netilmicin pH 7.0, 22°C Campylobacter jejuni

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.190 0.00016
-
 neamine pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.00073
-
 neomycin B pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.0085
-
 paromomycin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.012
-
 lividomycin A pH 7.0, 22°C Campylobacter jejuni

General Information

EC Number General Information Comment Organism
2.7.1.190 physiological function expression in Escherichia coli confers resistance to the 4,6-disubstituted aminoglycosides kanamycin, tobramycin, dibekacin, gentamicin, and sisomicin, but not to arbekacin, amikacin, isepamicin, or netilmicin, but not to any of the 4,5-disubstituted antibiotics tested Campylobacter jejuni

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.190 0.19
-
 ATP pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 0.45
-
 isepamicin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 25
-
 amikacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 480
-
 tobramycin above 480, pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 510
-
 GTP pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 660
-
 dibekacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 690
-
 kanamycin B pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 690
-
 arbekacin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 1500
-
 kanamycin A pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 1700
-
 netilmicin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 11000
-
 sisomicin pH 7.0, 22°C Campylobacter jejuni
2.7.1.190 12000
-
 gentamicin C pH 7.0, 22°C Campylobacter jejuni