EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.55 | functional recombinant expression of full-length enzyme and isolated catalytic module CtGH43 in Escherichia coli strain BL21(DE3) pLysS | Acetivibrio thermocellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.55 | Ag+ | 80% inhibition at 20 mM | Acetivibrio thermocellus | |
3.2.1.55 | Al3+ | 50% inhibition at 20 mM | Acetivibrio thermocellus | |
3.2.1.55 | Co2+ | 75% inhibition at 20 mM | Acetivibrio thermocellus | |
3.2.1.55 | Cu2+ | 70% inhibition at 10 mM | Acetivibrio thermocellus | |
3.2.1.55 | EDTA | 95% inhibition at 20 mM | Acetivibrio thermocellus | |
3.2.1.55 | EGTA | 95% inhibition at 20 mM | Acetivibrio thermocellus | |
3.2.1.55 | Fe3+ | 50% inhibition at 20 mM | Acetivibrio thermocellus | |
3.2.1.55 | Hg2+ | 80% inhibition at 20 mM | Acetivibrio thermocellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 0.04 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.05 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.072 | - |
soluble wheat arabinoxylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.078 | - |
soluble wheat arabinoxylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.08 | - |
oat spelt xylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.08 | - |
rye arabinoxylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.082 | - |
rye arabinoxylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.085 | - |
oat spelt xylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.09 | - |
insoluble wheat arabinoxylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.093 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.097 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.1 | - |
insoluble wheat arabinoxylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.7 | - |
beechwood xylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.8 | - |
beechwood xylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.9 | - |
birchwood xylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.95 | - |
birchwood xylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.55 | Ca2+ | activates the enzyme 2.16fold at 8 mM, presence of Ca2+ ions imparts thermal stability | Acetivibrio thermocellus | |
3.2.1.55 | Mg2+ | activates the enzyme 2.1fold at 6 mM | Acetivibrio thermocellus | |
3.2.1.55 | Mn2+ | activates the enzyme 1.2fold at 4 mM | Acetivibrio thermocellus | |
3.2.1.55 | Ni2+ | activates the enzyme 1.2fold at 4 mM | Acetivibrio thermocellus | |
3.2.1.55 | Zn2+ | activates the enzyme 1.2fold at 2 mM | Acetivibrio thermocellus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 34000 | - |
x * 63000, recombinant full-length enzyme, SDS-PAGE, x * 34000, recombinant catalytic module CtGH43 , SDS-PAGE | Acetivibrio thermocellus |
3.2.1.55 | 63000 | - |
x * 63000, recombinant full-length enzyme, SDS-PAGE, x * 34000, recombinant catalytic module CtGH43 , SDS-PAGE | Acetivibrio thermocellus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.55 | Acetivibrio thermocellus | A3DEX4 | two isozymes | - |
3.2.1.55 | Acetivibrio thermocellus ATCC 27405 | A3DEX4 | two isozymes | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 1.1 | - |
recombinant full-length enzyme, pH 5.7, 50°C, substrate insoluble wheat arabinoxylan | Acetivibrio thermocellus |
3.2.1.55 | 1.2 | - |
recombinant catalytic module CtGH43, pH 5.7, 50°C, substrate insoluble wheat arabinoxylan | Acetivibrio thermocellus |
3.2.1.55 | 1.7 | - |
recombinant full-length enzyme, pH 5.7, 50°C, substrate oat spelt xylan | Acetivibrio thermocellus |
3.2.1.55 | 1.8 | - |
recombinant catalytic module CtGH43, pH 5.7, 50°C, substrate oat spelt xylan | Acetivibrio thermocellus |
3.2.1.55 | 2.5 | - |
recombinant full-length enzyme, pH 5.7, 50°C, substrate soluble wheat arabinoxylan | Acetivibrio thermocellus |
3.2.1.55 | 2.7 | - |
recombinant catalytic module CtGH43, pH 5.7, 50°C, substrate soluble wheat arabinoxylan | Acetivibrio thermocellus |
3.2.1.55 | 4.7 | - |
recombinant full-length enzyme, pH 5.7, 50°C, substrate rye arabinoxylan | Acetivibrio thermocellus |
3.2.1.55 | 5 | - |
recombinant catalytic module CtGH43, pH 5.7, 50°C, substrate rye arabinoxylan | Acetivibrio thermocellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.55 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + L-arabinofuranose | - |
? | |
3.2.1.55 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Acetivibrio thermocellus ATCC 27405 | 4-nitrophenol + L-arabinofuranose | - |
? | |
3.2.1.55 | 4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + L-arabinopyranose | - |
? | |
3.2.1.55 | beechwood xylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
3.2.1.55 | beechwood xylan + H2O | - |
Acetivibrio thermocellus ATCC 27405 | ? | - |
? | |
3.2.1.55 | birchwood xylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
3.2.1.55 | birchwood xylan + H2O | - |
Acetivibrio thermocellus ATCC 27405 | ? | - |
? | |
3.2.1.55 | insoluble wheat arabinoxylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
3.2.1.55 | additional information | good substrates are rye and wheat arabinoxylan and oat spelt xylan with release of L-arabinose. Lower activity with xylan from beechwood and birchwood, arabinogalactan, arabinan from sugar beet, carboxy methyl cellulose and carboxy ethylcellulose | Acetivibrio thermocellus | ? | - |
? | |
3.2.1.55 | additional information | good substrates are rye and wheat arabinoxylan and oat spelt xylan with release of L-arabinose. Lower activity with xylan from beechwood and birchwood, arabinogalactan, arabinan from sugar beet, carboxy methyl cellulose and carboxy ethylcellulose | Acetivibrio thermocellus ATCC 27405 | ? | - |
? | |
3.2.1.55 | oat spelt xylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
3.2.1.55 | oat spelt xylan + H2O | - |
Acetivibrio thermocellus ATCC 27405 | ? | - |
? | |
3.2.1.55 | rye arabinoxylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
3.2.1.55 | soluble wheat arabinoxylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.55 | ? | x * 63000, recombinant full-length enzyme, SDS-PAGE, x * 34000, recombinant catalytic module CtGH43 , SDS-PAGE | Acetivibrio thermocellus |
3.2.1.55 | More | the enzyme displays an N-terminal catalytic module CtGH43 followed by two carbohydrate binding modules CtCBM6A and CtCBM6B towards the C-terminus. Circular dichroism analysis of catalytic domain CtGH43 shows 48% beta-sheets, 49% random coils but only 3% alpha-helices | Acetivibrio thermocellus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.55 | Ct43Araf | - |
Acetivibrio thermocellus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 50 | - |
- |
Acetivibrio thermocellus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 0.43 | - |
beechwood xylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 0.45 | - |
birchwood xylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.47 | - |
beechwood xylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 0.48 | - |
birchwood xylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 1.02 | - |
insoluble wheat arabinoxylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 1.05 | - |
insoluble wheat arabinoxylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 1.08 | - |
oat spelt xylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 1.12 | - |
oat spelt xylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 3.2 | - |
soluble wheat arabinoxylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 3.48 | - |
soluble wheat arabinoxylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 3.5 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 3.53 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 4.7 | - |
rye arabinoxylan | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 4.72 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.7, 50°C, recombinant full-length enzyme | Acetivibrio thermocellus | |
3.2.1.55 | 4.78 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus | |
3.2.1.55 | 4.97 | - |
rye arabinoxylan | pH 5.7, 50°C, recombinant catalytic module CtGH43 | Acetivibrio thermocellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 5.4 | - |
recombinant catalytic module CtGH43 | Acetivibrio thermocellus |
3.2.1.55 | 5.7 | - |
recombinant full-length enzyme | Acetivibrio thermocellus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.55 | evolution | the enzyme belongs to the family 43 glycoside hydrolase, GH43 | Acetivibrio thermocellus |
3.2.1.55 | additional information | the enzyme displays an N-terminal catalytic module CtGH43 followed by two carbohydrate binding modules CtCBM6A and CtCBM6B towards the C-terminus, CtCBM6A and CtCBM6B do not have any effect on the enzyme activity | Acetivibrio thermocellus |