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Literature summary extracted from

  • Motta, F.; Bastos, I.; Faudry, E.; Ebel, C.; Lima, M.; Neves, D.; Ragno, M.; Barbosa, J.; de Freitas, S.; Santana, J.
    The Trypanosoma cruzi virulence factor oligopeptidase B (OPBTc) assembles into an active and stable dimer (2012), PLoS ONE, 7, e30431.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.83 expression in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 additional information dithiothreitol is not inhibitoryup to 40 mM Trypanosoma cruzi
3.4.21.83 NaCl loss of almost 50 and 70% of its activity in the presence of 0.2 and 1.0 M NaCl, respectively. Presence of NaCl does not disrupt the dimeric structure Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 80000
-
 2 * 80000, SDS-PAGE Trypanosoma cruzi
3.4.21.83 120000
-
 PAGE Trypanosoma cruzi
3.4.21.83 189000
-
 gel filtration Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Trypanosoma cruzi
-
-
-
3.4.21.83 Trypanosoma cruzi Brener
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi Brener benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.83 dimer 2 * 80000, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.4.21.83 OPBTc
-
 Trypanosoma cruzi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.83 42
-
 enzyme retains its dimeric structure and is fully active up to 42°C Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.83 8
-
-
 Trypanosoma cruzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.83 7
-
 about 80% of maximum activity Trypanosoma cruzi