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Literature summary extracted from

  • Moreno-Pérez, A.; Venegas-Calerón, M.; Vaistij, F.; Salas, J.; Larson, T.; Garcés, R.; Graham, I.; Martínez-Force, E.
    Effect of a mutagenized acyl-ACP thioesterase FATA allele from sunflower with improved activity in tobacco leaves and Arabidopsis seeds (2014), Planta, 239, 669-677.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 expressed in Tobacco, Arabidopsis thaliana and Escherichia coli Helianthus annuus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.14 L118W mutant displays three orders of magnitude lower catalytic activity compared to wild-type. Km value similar to wild-type Helianthus annuus
3.1.2.14 M206W mutant displays lower catalytic activity compared to wild-type. Km value similar to wild-type, only for substrate 18:1-ACP Km value highly increased compared to wild-type Helianthus annuus
3.1.2.14 Q215W mutant displays equivalent or improved activities towards all substrates with marked 2 and 2.6fold increased activities for palmitoleoyl-ACP and palmitoyl-ACP, respectively, compared to wild-type. Expression of mutant in Nicotiana benthamiana leads to a higher average values of acyl-CoA. Expressed in Arabidopsis mutant displays displays a 40% decrease in oil content relative to wild-type. Mutant produces an increase in the content of saturated fatty acids, palmitic and stearic acids, with a concomitant decrease in oleic acid derivatives Helianthus annuus
3.1.2.14 T182W mutant displays lower catalytic activity compared to wild-type. Km value similar to wild-type. Leaf triacylglycerols are increased 2 to 4fold when expressed in Nicotiana benthamiana compared to expression of mutant T182W and wild-type, respectively Helianthus annuus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.14 0.0001
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 0.0002
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 0.0003
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 0.0003
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 0.0004
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 0.0006
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 0.0006
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 0.0006
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 0.0007
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 0.0007
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 0.0009
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 0.0009
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 0.0009
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 0.001
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 0.001
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 0.0012
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 0.0015
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 0.0022
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 0.0025
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 0.0212
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant M206W Helianthus annuus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.14 37000
-
 SDS-PAGE Helianthus annuus

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Helianthus annuus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.14 using metal affinity chromatography Helianthus annuus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 oleoyl-[acyl-carrier-protein] + H2O
-
 Helianthus annuus oleic acid + acyl-carrier-protein
-
 ?
3.1.2.14 palmitoleoyl-[acyl-carrier protein] + H2O
-
 Helianthus annuus palmitoleate + acyl-carrier protein
-
 ?
3.1.2.14 palmitoyl-[acyl-carrier protein] + H2O
-
 Helianthus annuus palmitic acid + acyl-carrier protein
-
 ?
3.1.2.14 stearoyl-[acyl-carrier protein] + H2O
-
 Helianthus annuus stearic acid + acyl-carrier protein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 HaFatA
-
 Helianthus annuus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.14 20
-
 assay at Helianthus annuus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.14 0.0002
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 0.0005
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 0.0016
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 0.002
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 0.0024
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 0.003
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 0.0041
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 0.0062
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 0.016
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 0.032
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 0.0854
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 0.1258
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 0.2
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 0.2
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 0.6
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 0.8
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 1.1
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 2.2
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 8
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 35.6
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, wild-type Helianthus annuus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.14 8
-
 assay at Helianthus annuus

General Information

EC Number General Information Comment Organism
3.1.2.14 malfunction null, wild type and high-efficiency alleles are transiently expressed in tobacco leaves to check their effect on lipid biosynthesis. Expression of active FatA thioesterases alters the composition of leaf triacylglycerols but does not alter total lipid content. The expression of the wild type and the high-efficiency alleles in Arabidopsis thaliana transgenic seeds results in a strong reduction in oil content and an increase in total saturated fatty acid content Helianthus annuus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.14 2
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 3
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 28
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 50
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant T182W Helianthus annuus
3.1.2.14 200
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 300
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 400
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 500
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 1100
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 1200
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 1600
-
 stearoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 1800
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 2800
-
 palmitoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 3700
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant L118W Helianthus annuus
3.1.2.14 5900
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 6600
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant M206W Helianthus annuus
3.1.2.14 23400
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 39000
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, wild-type Helianthus annuus
3.1.2.14 39300
-
 oleoyl-[acyl-carrier-protein] pH 8, 20°C, mutant Q215W Helianthus annuus
3.1.2.14 48000
-
 palmitoleoyl-[acyl-carrier protein] pH 8, 20°C, mutant Q215W Helianthus annuus