BRENDA - Enzyme Database

Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity

Dyer, J.M.; Chapital, D.C.; Kuan, J.C.; Mullen, R.T.; Turner, C.; McKeon, T.A.; Pepperman, A.B.; Plant Physiol. 130, 2027-2038 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.14
expressed in Nicotiana tabacum BY-2 cells and in Saccharomyces cerevisiae
Vernicia fordii
1.14.19.33
expression in Saccharomyces cerevisiae; gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11, transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
Vernicia fordii
1.14.19.34
gene FAD2, DNA and amino acid sequence determination and analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11,transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
Vernicia fordii
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.14
endoplasmic reticulum
-
Vernicia fordii
5783
-
1.14.19.33
endoplasmic reticulum
-
Vernicia fordii
5783
-
1.14.19.34
endoplasmic reticulum
-
Vernicia fordii
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.33
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
the enzyme is involved in the biosynthetic pathway of eleostearic acid
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
-
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
the enzyme is involved in the biosynthetic pathway of eleostearic acid
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
?
1.14.19.34
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
-
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.14
Vernicia fordii
Q8GZC2
-
-
1.14.19.33
Vernicia fordii
Q8GZC2
-
-
1.14.19.34
Vernicia fordii
Q8GZC3
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.14
additional information
not detected in seed
Vernicia fordii
-
1.14.19.14
seed
-
Vernicia fordii
-
1.14.19.33
seed
expressed exclusively within developing seeds; FADX is expressed exclusively in developing tung seeds
Vernicia fordii
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.14
(6Z,9Z,12Z)-octadec-6,9,12-trienoyl-[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(6Z,9Z,11E,13E)-octadec-6,9,11,13-tetraenoyl -[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
(9Z)-hexadec-9-enoyl -[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(9Z,12E)-hexadec-9,12-dienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
(9Z)-octadec-9-enoyl-[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(9Z,12E)-octadec-9,12-dienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(9Z,11Z,13Z)-octadec-9,12,13-trienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
(9Z,12Z,15Z)-octadec-9,12,15-trienoyl-[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(9Z,11E,13E,15Z)-octadec-9,11,13,15-tetraenoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
additional information
FADX is a bifunctional enzyme with robust desaturase/conjugase activity
732650
Vernicia fordii
?
-
-
-
-
1.14.19.33
(6Z,9Z,12Z)-octadeca-6,9,12-trienoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(6Z,9Z,11E,13E)-octadeca-6,9,11,13-tetraenoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
(9Z)-hexadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(9Z,12E)-hexadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
(9Z)-octadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(9Z,12E)-octadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
-
?
1.14.19.33
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the enzyme is involved in the biosynthetic pathway of eleostearic acid
732650
Vernicia fordii
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the enzyme is involved in the biosynthetic pathway of eleostearic acid
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the double bond introduced by FADX during fatty acid desaturation is in the trans, rather than cis, configuration
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
additional information
the enzyme is capable of generating a variety of alternative conjugated and 12-desaturated fatty acid products in yeast cells cultured in the presence of exogenously supplied fatty acid substrates
732650
Vernicia fordii
?
-
-
-
-
1.14.19.33
additional information
a bifunctional enzyme also exhibiting the activity of EC 1.14.19.34
732650
Vernicia fordii
?
-
-
-
-
1.14.19.34
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.34
additional information
a bifunctional enzyme also exhibiting the activity of EC 1.14.19.33
732650
Vernicia fordii
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.33
cytochrome b5
-
Vernicia fordii
1.14.19.34
cytochrome b5
-
Vernicia fordii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.14
expressed in Nicotiana tabacum BY-2 cells and in Saccharomyces cerevisiae
Vernicia fordii
1.14.19.33
expression in Saccharomyces cerevisiae; gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11, transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
Vernicia fordii
1.14.19.34
gene FAD2, DNA and amino acid sequence determination and analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11,transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
Vernicia fordii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.33
cytochrome b5
-
Vernicia fordii
1.14.19.34
cytochrome b5
-
Vernicia fordii
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.14
endoplasmic reticulum
-
Vernicia fordii
5783
-
1.14.19.33
endoplasmic reticulum
-
Vernicia fordii
5783
-
1.14.19.34
endoplasmic reticulum
-
Vernicia fordii
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.33
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
the enzyme is involved in the biosynthetic pathway of eleostearic acid
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
-
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
the enzyme is involved in the biosynthetic pathway of eleostearic acid
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
?
1.14.19.34
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
-
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.14
additional information
not detected in seed
Vernicia fordii
-
1.14.19.14
seed
-
Vernicia fordii
-
1.14.19.33
seed
expressed exclusively within developing seeds; FADX is expressed exclusively in developing tung seeds
Vernicia fordii
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.14
(6Z,9Z,12Z)-octadec-6,9,12-trienoyl-[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(6Z,9Z,11E,13E)-octadec-6,9,11,13-tetraenoyl -[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
(9Z)-hexadec-9-enoyl -[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(9Z,12E)-hexadec-9,12-dienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
(9Z)-octadec-9-enoyl-[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(9Z,12E)-octadec-9,12-dienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(9Z,11Z,13Z)-octadec-9,12,13-trienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
(9Z,12Z,15Z)-octadec-9,12,15-trienoyl-[glycerolipid] + reduced acceptor + O2
-
732650
Vernicia fordii
(9Z,11E,13E,15Z)-octadec-9,11,13,15-tetraenoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
1.14.19.14
additional information
FADX is a bifunctional enzyme with robust desaturase/conjugase activity
732650
Vernicia fordii
?
-
-
-
-
1.14.19.33
(6Z,9Z,12Z)-octadeca-6,9,12-trienoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(6Z,9Z,11E,13E)-octadeca-6,9,11,13-tetraenoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
(9Z)-hexadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(9Z,12E)-hexadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
(9Z)-octadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(9Z,12E)-octadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
-
?
1.14.19.33
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the enzyme is involved in the biosynthetic pathway of eleostearic acid
732650
Vernicia fordii
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the enzyme is involved in the biosynthetic pathway of eleostearic acid
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
-
?
1.14.19.33
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the double bond introduced by FADX during fatty acid desaturation is in the trans, rather than cis, configuration
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.33
additional information
the enzyme is capable of generating a variety of alternative conjugated and 12-desaturated fatty acid products in yeast cells cultured in the presence of exogenously supplied fatty acid substrates
732650
Vernicia fordii
?
-
-
-
-
1.14.19.33
additional information
a bifunctional enzyme also exhibiting the activity of EC 1.14.19.34
732650
Vernicia fordii
?
-
-
-
-
1.14.19.34
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.34
additional information
a bifunctional enzyme also exhibiting the activity of EC 1.14.19.33
732650
Vernicia fordii
?
-
-
-
-
General Information
EC Number
General Information
Commentary
Organism
1.14.19.14
physiological function
enzyme expression is occasionally associated with dramatic changes in morphology of the endoplasmic reticulum
Vernicia fordii
1.14.19.33
evolution
tung FADX is grouped with DELTA12 fatty acid desaturases and hydroxylases rather than conjugases, which is consistent with its desaturase activity
Vernicia fordii
1.14.19.33
additional information
incubation of yeast cells expressing FADX with exogenously supplied palmitoleicacid leads to substantial incorporation of thisfatty acid into yeast cells, accounting for approximately79% of total fatty acids. Oleic acid is reduced to only 3% under these growth conditions
Vernicia fordii
1.14.19.33
physiological function
FADX converts linoleic acid into alpha-eleostearic acid. in the plant seed oil
Vernicia fordii
1.14.19.34
evolution
the enzyme belongs to the FAD2 enzyme family
Vernicia fordii
1.14.19.34
physiological function
FAD2 converts oleic acid into linoleic acid in the plant seed oil
Vernicia fordii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.19.14
physiological function
enzyme expression is occasionally associated with dramatic changes in morphology of the endoplasmic reticulum
Vernicia fordii
1.14.19.33
evolution
tung FADX is grouped with DELTA12 fatty acid desaturases and hydroxylases rather than conjugases, which is consistent with its desaturase activity
Vernicia fordii
1.14.19.33
additional information
incubation of yeast cells expressing FADX with exogenously supplied palmitoleicacid leads to substantial incorporation of thisfatty acid into yeast cells, accounting for approximately79% of total fatty acids. Oleic acid is reduced to only 3% under these growth conditions
Vernicia fordii
1.14.19.33
physiological function
FADX converts linoleic acid into alpha-eleostearic acid. in the plant seed oil
Vernicia fordii
1.14.19.34
evolution
the enzyme belongs to the FAD2 enzyme family
Vernicia fordii
1.14.19.34
physiological function
FAD2 converts oleic acid into linoleic acid in the plant seed oil
Vernicia fordii