BRENDA - Enzyme Database

Functional characterization of a plastidial omega-3 desaturase from sunflower (Helianthus annuus) in cyanobacteria

Venegas-Caleron, M.; Muro-Pastor, A.M.; Garces, R.; Martinez-Force, E.; Plant Physiol. Biochem. 44, 517-525 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.35
heterologous expression in Synechocystis sp. PCC 6803
Helianthus annuus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.35
chloroplast
-
Helianthus annuus
9507
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.35
50800
-
x * 50800, calculated from sequence. The proteolytic processing of the prepeptide would produce a 392 amino acid protein with a molecular mass of around 452000 Da
Helianthus annuus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.35
Helianthus annuus
Q56VS4
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.35
cotyledon
-
Helianthus annuus
-
1.14.19.35
leaf
-
Helianthus annuus
-
1.14.19.35
additional information
no or little expression is detected in mature seeds, roots and stems
Helianthus annuus
-
1.14.19.35
root
low expression level
Helianthus annuus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.35
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin + O2 + 2 H+
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]
732642
Helianthus annuus
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin + 2 H2O
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. alpha-linolenoyl-[glycerolipid]
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.35
?
x * 50800, calculated from sequence. The proteolytic processing of the prepeptide would produce a 392 amino acid protein with a molecular mass of around 452000 Da
Helianthus annuus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.19.35
Helianthus annuus
calculated from sequence. The proteolytic processing of the prepeptide would produce a 392 amino acid protein with a pI of 7.3
-
8.06
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.35
heterologous expression in Synechocystis sp. PCC 6803
Helianthus annuus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.35
chloroplast
-
Helianthus annuus
9507
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.35
50800
-
x * 50800, calculated from sequence. The proteolytic processing of the prepeptide would produce a 392 amino acid protein with a molecular mass of around 452000 Da
Helianthus annuus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.35
cotyledon
-
Helianthus annuus
-
1.14.19.35
leaf
-
Helianthus annuus
-
1.14.19.35
additional information
no or little expression is detected in mature seeds, roots and stems
Helianthus annuus
-
1.14.19.35
root
low expression level
Helianthus annuus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.35
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin + O2 + 2 H+
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]
732642
Helianthus annuus
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin + 2 H2O
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. alpha-linolenoyl-[glycerolipid]
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.35
?
x * 50800, calculated from sequence. The proteolytic processing of the prepeptide would produce a 392 amino acid protein with a molecular mass of around 452000 Da
Helianthus annuus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.19.35
Helianthus annuus
calculated from sequence. The proteolytic processing of the prepeptide would produce a 392 amino acid protein with a pI of 7.3
-
8.06