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Literature summary extracted from

  • Nafi, A.; Hooi Ling, F.; Bakar, J.; Ghazali, H.M.
    Partial characterization of an enzymatic Extract from Bentong Ginger (Zingiber officinale var. Bentong) (2014), Molecules, 19, 12336-12348.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.67 additional information no activating or inhibitory, but a stabilizing effect by EDTA Zingiber officinale
3.4.22.67 SDS enhances by 27.4% at 0.25 mM Zingiber officinale

General Stability

EC Number General Stability Organism
3.4.22.67 ascorbic acid, EDTA, and cysteine stabilize the purified enzyme Zingiber officinale
3.4.22.67 pH 7.0, potassium phosphate buffer and 10 mM cysteine in combination with 5 mM EDTA as stabilizer are most effective conditions during enzyme extraction Zingiber officinale

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.67 Al3+ slight inhibition Zingiber officinale
3.4.22.67 Ba2+ slight inhibition Zingiber officinale
3.4.22.67 Ca2+ slight inhibition Zingiber officinale
3.4.22.67 Co2+
-
 Zingiber officinale
3.4.22.67 Cu2+ strong inhibition Zingiber officinale
3.4.22.67 Hg2+ complete inhibition Zingiber officinale
3.4.22.67 Li+
-
 Zingiber officinale
3.4.22.67 Mg2+ slight inhibition Zingiber officinale
3.4.22.67 additional information no activating or inhibitory, but a stabilizing effect by EDTA, no inhibition by K+ and Na+ Zingiber officinale
3.4.22.67 NEM strong inhibition Zingiber officinale
3.4.22.67 o-phenanthroline
-
 Zingiber officinale
3.4.22.67 PMSF
-
 Zingiber officinale
3.4.22.67 Tween 20
-
 Zingiber officinale
3.4.22.67 Tween 80
-
 Zingiber officinale
3.4.22.67 Zn2+
-
 Zingiber officinale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.67 additional information
-
 additional information Km and Vmax values are 0.21 mg/ml and 34.48 mg/m/min, respectively, with substrate azocasein Zingiber officinale

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.67 Zingiber officinale
-
 var. Bentong, from Malaysia
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.67 rhizome
-
 Zingiber officinale
-

Storage Stability

EC Number Storage Stability Organism
3.4.22.67 ?20°C, purified lyophilized enzyme, retains activity for 22 months Zingiber officinale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.67 azocasein + H2O
-
 Zingiber officinale ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.67 Bgp
-
 Zingiber officinale

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.67 60
-
-
 Zingiber officinale

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.67 25 80 activity range, profile overview Zingiber officinale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.67 6 8
-
 Zingiber officinale

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.67 4.5 9.5 activity range, profile overview Zingiber officinale