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Literature summary extracted from

  • Banoczi, Z.; Tantos, A.; Farkas, A.; Majer, Z.; Dokus, L.E.; Tompa, P.; Hudecz, F.
    New m-calpain substrate-based azapeptide inhibitors (2013), J. Pept. Sci., 19, 370-376.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.53 expressed in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.53 Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-NH2
-
 Rattus norvegicus
3.4.22.53 Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2
-
 Rattus norvegicus
3.4.22.53 Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2
-
 Rattus norvegicus
3.4.22.53 Ac-Thr-Ser-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2
-
 Rattus norvegicus
3.4.22.53 Ac-Thr-Trp-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2
-
 Rattus norvegicus
3.4.22.53 additional information not inhibited by Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-NH2, Ac-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Thr-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Ser-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Gln-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Val-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Thr-Pro-Pro-Pro-Ser-NH2, and Ac-Thr-Pro-Leu-alpha-azaglycine-Arg-Pro-Pro-Pro-Ser-NH2 Rattus norvegicus
3.4.22.53 Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.53 Ca2+ 3 mM used in assay conditions Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.53 80000
-
 x * 80000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.53 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.53 HisTrap column chromatography Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.53 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.22.53 TPLKSPPPSPR + H2O efficient substrate Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.53 ? x * 80000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.53 calpain 2
-
 Rattus norvegicus
3.4.22.53 m-calpain
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.53 0.0035
-
 Ac-Thr-Ser-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
3.4.22.53 0.0058
-
 Ac-Thr-Trp-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
3.4.22.53 0.0072
-
 Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
3.4.22.53 0.0087
-
 Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
3.4.22.53 0.0203
-
 Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
3.4.22.53 0.0898
-
 Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus