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Literature summary extracted from

  • Shin, K.C.; Lee, G.W.; Oh, D.K.
    Production of ginsenoside Rd from ginsenoside Rc by alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus (2013), J. Microbiol. Biotechnol., 23, 483-488.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 additional information no significant effect by 5-10% v/v toluene Caldicellulosiruptor saccharolyticus
3.2.1.55 n-hexane activates about 20% at 5-10% v/v, and 230% at 30% v/v Caldicellulosiruptor saccharolyticus

Application

EC Number Application Comment Organism
3.2.1.55 synthesis the thermostable enzyme is used for production of ginsenoside Rd from ginsenoside Rc, optimal reaction conditions are pH 5.5, 80°C, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% v/v n-hexane Caldicellulosiruptor saccharolyticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 recombinant expression of the enzyme in Escherichia coli Caldicellulosiruptor saccharolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 3-methyl-1-butanol inhibits 80% and 97% at 5% and 10% v/v, respectively Caldicellulosiruptor saccharolyticus
3.2.1.55 acetone inhibits 15% and 50% at 5% and 10% v/v, respectively Caldicellulosiruptor saccharolyticus
3.2.1.55 DMSO inhibits 10% and 40% at 5% and 10% v/v, respectively Caldicellulosiruptor saccharolyticus
3.2.1.55 ethanol inhibits 25% and 55% at 5% and 10% v/v, respectively Caldicellulosiruptor saccharolyticus
3.2.1.55 methanol inhibits 15% and 50% at 5% and 10% v/v, respectively Caldicellulosiruptor saccharolyticus
3.2.1.55 additional information no significant effect by 5-10% v/v toluene Caldicellulosiruptor saccharolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 57882
-
 8 * 57882, sequence calculation, 8 * 58000, recombinant enzyme, SDS-PAGE Caldicellulosiruptor saccharolyticus
3.2.1.55 58000
-
 8 * 57882, sequence calculation, 8 * 58000, recombinant enzyme, SDS-PAGE Caldicellulosiruptor saccharolyticus
3.2.1.55 460000
-
 recombinant enzyme, gel filtration Caldicellulosiruptor saccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Caldicellulosiruptor saccharolyticus A4XJR8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 recombinant enzyme 25.6fold from Escherichia coli by heat treatment and anion exchange chromatography Caldicellulosiruptor saccharolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 75.7
-
 purified recombinant enzyme, pH 5.5, 85°C Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 ginsenoside Mc + H2O
-
 Caldicellulosiruptor saccharolyticus ginsenoside K + L-arabinofuranose
-
 ?
3.2.1.55 ginsenoside Rc + H2O thermostable enzyme produces ginsenoside Rd from ginsenoside Rc, optimal reaction conditions are pH 5.5, 80°C, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% v/v n-hexane. The enzyme completely converts ginsenoside Rc to ginsenoside Rd with very high productivity Caldicellulosiruptor saccharolyticus ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.55 additional information substrate specificity, overview. No activity with 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-L-arabinofuranoside, 4-nitrophenyl-beta-L-arabinopyranoside 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, ginsenoside Rb2, which contains alpha-L-arabinopyranoside at C-20, or ginsenoside Rb1, which contains beta-D-glucopyranoside at C-20 Caldicellulosiruptor saccharolyticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 octamer 8 * 57882, sequence calculation, 8 * 58000, recombinant enzyme, SDS-PAGE Caldicellulosiruptor saccharolyticus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
 Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 85
-
-
 Caldicellulosiruptor saccharolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 40 90 over 40% activity within this range, profile overview Caldicellulosiruptor saccharolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 70 85 thermal inactivation of the purified enzyme follows first-order kinetics with half-lives of 130, 41, 2.5, and 0.12 h at 70°C, 75°C, 80°C, and 85°C, respectively Caldicellulosiruptor saccharolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5.5
-
-
 Caldicellulosiruptor saccharolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4.5 7 activity range, profile overview Caldicellulosiruptor saccharolyticus