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Literature summary extracted from

  • Kang, C.; Yu, X.W.; Xu, Y.
    Cloning and expression of a novel prolyl endopeptidase from Aspergillus oryzae and its application in beer stabilization (2015), J. Ind. Microbiol. Biotechnol., 42, 263-272.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.26 expressed in Pichia pastoris strain GS115 Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.26 Al3+ 34.5% residual activity at 1 mM Aspergillus oryzae
3.4.21.26 Cu2+ 14.1% residual activity at 1 mM Aspergillus oryzae
3.4.21.26 Fe2+ 80.6% residual activity at 1 mM Aspergillus oryzae
3.4.21.26 K+ 92.2% residual activity at 1 mM Aspergillus oryzae
3.4.21.26 Mg2+ 87.3% residual activity at 1 mM Aspergillus oryzae
3.4.21.26 Mn2+ 24.3% residual activity at 1 mM Aspergillus oryzae
3.4.21.26 Na+ 93.1% residual activity at 1 mM Aspergillus oryzae
3.4.21.26 Zn2+ 31.2% residual activity at 1 mM Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.26 0.28
-
 L-Ala-L-Ala-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae
3.4.21.26 0.37
-
 Z-Gly-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae
3.4.21.26 0.56
-
 Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.26 Ca2+ Ca2+ shows activating effect on enzyme activity Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.26 64000
-
 x * 64000, SDS-PAGE Aspergillus oryzae
3.4.21.26 64800
-
 x * 64800, calculated from amino acid sequence Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.26 Aspergillus oryzae
-
-
-
3.4.21.26 Aspergillus oryzae WX2011
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.26 HiTrap DEAE column chromatography Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.26 L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.21.26 L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae WX2011 L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.21.26 Z-Gly-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae Z-Gly-L-Pro + 4-nitroaniline
-
 ?
3.4.21.26 Z-Gly-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae WX2011 Z-Gly-L-Pro + 4-nitroaniline
-
 ?
3.4.21.26 Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae Z-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.21.26 Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae WX2011 Z-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.26 ? x * 64000, SDS-PAGE Aspergillus oryzae
3.4.21.26 ? x * 64800, calculated from amino acid sequence Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.4.21.26 PEP
-
 Aspergillus oryzae
3.4.21.26 prolyl endopeptidase
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.26 40
-
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.26 40 80 the enzyme retains over 80% activity after incubation for 30 min at 40°C. The enzyme is not stable at 65 and 70°C, decreasing to 40 and 32% maximum activity, respectively. The activity is almost lost after incubation of 30 min at 80°C Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.26 93
-
 Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae
3.4.21.26 105.7
-
 Z-Gly-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae
3.4.21.26 139.4
-
 L-Ala-L-Ala-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.26 5
-
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.26 3 8 over 60% of the maximum activity is retained between pH 3.0 and 8.0 Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.26 3.5 7 within the pH range of 3.5-7.0, the enzyme retains more than 80% of the initial activity after incubation at 37°C for 30 min Aspergillus oryzae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.26 Aspergillus oryzae calculated from amino acid sequence
-
 5.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.26 166.1
-
 Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae
3.4.21.26 285.7
-
 Z-Gly-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae
3.4.21.26 496.4
-
 L-Ala-L-Ala-L-Pro-4-nitroanilide at 40°C and pH 4.0 Aspergillus oryzae