Literature summary extracted from
Unciti-Broceta, J.D.; Maceira, J.; Morales, S.; Garcia-Perez, A.; Munoz-Torres, M.E.; Garcia-Salcedo, J.A.
Nicotinamide inhibits the lysosomal cathepsin B-like protease and kills African trypanosomes (2013), J. Biol. Chem., 288, 10548-10557.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.22.B6 |
recombinant expression in Pichia pastoris |
Trypanosoma brucei |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.22.B6 |
nicotinamide |
complete inhibition at 25 mM |
Trypanosoma brucei |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.4.22.B6 |
lysosome |
- |
Trypanosoma brucei |
5764 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.4.22.B6 |
42000 |
- |
x * 42000, recombinant zymogen, SDS-PAGE |
Trypanosoma brucei |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.22.B6 |
Trypanosoma brucei |
Q6R7Z5 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.22.B6 |
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O |
- |
Trypanosoma brucei |
Z-Arg-Arg + 7-amino-4-methylcoumarin |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.22.B6 |
? |
x * 42000, recombinant zymogen, SDS-PAGE |
Trypanosoma brucei |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.22.B6 |
CatB |
- |
Trypanosoma brucei |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.22.B6 |
37 |
- |
assay at |
Trypanosoma brucei |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.22.B6 |
6 |
- |
assay at |
Trypanosoma brucei |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.4.22.B6 |
malfunction |
inhibition of the lysosomal cathepsin B-like protease blocks endocytosis causing cell death. Nicotinamide causes cytokinesis defects and cell cycle arrest at G2 phase |
Trypanosoma brucei |
3.4.22.B6 |
physiological function |
cathepsin B-like is a lysosomal protease responsible for degradation of host-derived growth factors, such as transferrin, in the bloodstream form of Trypanosoma brucei |
Trypanosoma brucei |