BRENDA - Enzyme Database show

Aromatic residues in the substrate cleft of RPE65 protein govern retinol isomerization and modulate its progression

Chander, P.; Gentleman, S.; Poliakov, E.; Redmond, T.; J. Biol. Chem. 287, 30552-30559 (2012)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.90
F103I
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F103L
mutant produces increased amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F103W
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F103Y
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F312L
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F312W
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F312Y
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F418A
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F418L
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F418W
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F418Y
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F526A
mutant shows a increased production level of 13-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F526L
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F526W
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F526Y
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F61A
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F61L
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F61W
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F61Y
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
Y338A
mutant produces increased amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
Y338F
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
Y338L
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
Y338W
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.90
Mus musculus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.90
all-trans retinyl ester + H2O
-
732066
Mus musculus
13-cis retinol + ?
-
-
-
-
3.1.1.90
all-trans retinyl ester + H2O
-
732066
Mus musculus
11-cis retinol + ?
-
-
-
-
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.90
F103I
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F103L
mutant produces increased amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F103W
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F103Y
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F312L
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F312W
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F312Y
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F418A
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F418L
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F418W
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F418Y
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F526A
mutant shows a increased production level of 13-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F526L
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F526W
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F526Y
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
F61A
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F61L
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F61W
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
F61Y
mutant shows severly impaired enzymatic activity
Mus musculus
3.1.1.90
Y338A
mutant produces increased amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
Y338F
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
Y338L
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
3.1.1.90
Y338W
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.90
all-trans retinyl ester + H2O
-
732066
Mus musculus
13-cis retinol + ?
-
-
-
-
3.1.1.90
all-trans retinyl ester + H2O
-
732066
Mus musculus
11-cis retinol + ?
-
-
-
-