Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, S.; Huang, H.; Moll, J.; Thauer, R.K.
    NADP+ reduction with reduced ferredoxin and NADP+ reduction with NADH are coupled via an electron-bifurcating enzyme complex in Clostridium kluyveri (2010), J. Bacteriol., 192, 5115-5123.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.1.4 expression in Escherichia coli Clostridium kluyveri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.1.4 0.025
-
 NADPH pH 7, 37°C Clostridium kluyveri
1.6.1.4 1
-
 NAD+ pH 7, 37°C Clostridium kluyveri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.109 cytoplasm
-
 Clostridium kluyveri 5737
-
1.6.1.4 cytoplasm
-
 Clostridium kluyveri 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.109 (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster Clostridium kluyveri
-
 butanoyl-CoA + NAD+ + reduced ferredoxin [iron-sulfur] cluster
-
 ?
1.3.1.109 (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster Clostridium kluyveri DSM 555
-
 butanoyl-CoA + NAD+ + reduced ferredoxin [iron-sulfur] cluster
-
 ?
1.6.1.4 NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster Clostridium kluyveri the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster
-
 r
1.6.1.4 NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster Clostridium kluyveri DSM 555 the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.109 Clostridium kluyveri
-
-
-
1.3.1.109 Clostridium kluyveri DSM 555
-
-
-
1.6.1.4 Clostridium kluyveri A5N5D2 and A5N5D3 A5N5D2: NfnA, A5N5D3: NfnB
-
1.6.1.4 Clostridium kluyveri DSM 555 A5N5D2 and A5N5D3 A5N5D2: NfnA, A5N5D3: NfnB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.1.4
-
 Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.109 (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster
-
 Clostridium kluyveri butanoyl-CoA + NAD+ + reduced ferredoxin [iron-sulfur] cluster
-
 ?
1.3.1.109 (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster
-
 Clostridium kluyveri DSM 555 butanoyl-CoA + NAD+ + reduced ferredoxin [iron-sulfur] cluster
-
 ?
1.6.1.4 NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster in the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+). In the absence of ferredoxin, NAD+ is only very slowly reduced (less than 1% of the rate observed in the presence of ferredoxin) Clostridium kluyveri NADH + H+ + 2 NADP+ + reduced ferredoxin iron-sulfur cluster
-
 r
1.6.1.4 NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster in the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+). In the absence of ferredoxin, NAD+ is only very slowly reduced (less than 1% of the rate observed in the presence of ferredoxin) Clostridium kluyveri DSM 555 NADH + H+ + 2 NADP+ + reduced ferredoxin iron-sulfur cluster
-
 r
1.6.1.4 NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH Clostridium kluyveri NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster
-
 r
1.6.1.4 NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH Clostridium kluyveri DSM 555 NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster
-
 r
1.6.1.4 NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH. In the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+) Clostridium kluyveri NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster
-
 r
1.6.1.4 NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH. In the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+) Clostridium kluyveri DSM 555 NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.1.4 heterodimer
-
 Clostridium kluyveri

Synonyms

EC Number Synonyms Comment Organism
1.3.1.109 butyryl-CoA dehydrogenase-EtfAB complex
-
 Clostridium kluyveri
1.3.1.109 butyryl-coenzyme A dehydrogenase-EtfAB complex
-
 Clostridium kluyveri
1.6.1.4 NADH-dependent reduced ferredoxin:NADP+ oxidoreductase
-
 Clostridium kluyveri
1.6.1.4 NfnAB
-
 Clostridium kluyveri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.1.4 37
-
 assay at Clostridium kluyveri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.1.4 7
-
-
 Clostridium kluyveri

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.109 NADH
-
 Clostridium kluyveri
1.6.1.4 FAD iron-sulfur flavoprotein complex. Subunit NfnB contains FAD Clostridium kluyveri
1.6.1.4 iron-sulfur centre iron-sulfur flavoprotein complex, subunit NfnA has a predicted [2Fe2S] binding site, subunit NfnB has two predicted [4Fe4S] binding sites. UV-visible spectrum shows that the NfnAB complex contains up to 10 Fe molecules per heterodimer, which is consistent with the presence of two [4Fe4S] and one [2Fe2S] clusters Clostridium kluyveri