EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.133 | expressed in Escherichia coli BL21(DE3) cells | Geobacillus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.133 | Al3+ | about 3.0% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Ba2+ | about 0.5% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Ca2+ | about 32% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Co2+ | about 1.1% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Cr3+ | about 22.2% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | EDTA | complete inhibition at 10 mM | Geobacillus sp. | |
3.2.1.133 | Fe2+ | about 0.4% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Hg2+ | about 2.7% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Li+ | about 41% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Mg2+ | about 1.4% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Mn2+ | about 1.5% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Na+ | about 1.7% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Ni2+ | about 0.7% residual activity at 10 mM | Geobacillus sp. | |
3.2.1.133 | Sn4+ | about 89.3% residual activity at 10 mM | Geobacillus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.133 | Ca2+ | about 143.8% activity at 1 mM | Geobacillus sp. | |
3.2.1.133 | K+ | about 186.6% activity at 1 mM | Geobacillus sp. | |
3.2.1.133 | Li+ | about 166.5% activity at 1 mM | Geobacillus sp. | |
3.2.1.133 | Sn4+ | about 121% activity at 1 mM | Geobacillus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 65000 | - |
x * 65000, SDS-PAGE | Geobacillus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.133 | Geobacillus sp. | E0X988 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.133 | Ni-NTA agarose column chromatography | Geobacillus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.133 | alpha-cyclodextrin + H2O | high specificity for alpha-cyclodextrin | Geobacillus sp. | ? | - |
? | |
3.2.1.133 | amylopectin + H2O | - |
Geobacillus sp. | ? | - |
? | |
3.2.1.133 | amylose + H2O | - |
Geobacillus sp. | ? | - |
? | |
3.2.1.133 | beta-cyclodextrin + H2O | - |
Geobacillus sp. | ? | - |
? | |
3.2.1.133 | gamma-cyclodextrin + H2O | - |
Geobacillus sp. | ? | - |
? | |
3.2.1.133 | glycogen + H2O | - |
Geobacillus sp. | ? | - |
? | |
3.2.1.133 | starch + H2O | - |
Geobacillus sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.133 | ? | x * 65000, SDS-PAGE | Geobacillus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.133 | MAase | - |
Geobacillus sp. |
3.2.1.133 | maltogenic amylase | - |
Geobacillus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 55 | 65 | - |
Geobacillus sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 40 | 70 | about 40% activity at 40°C, about 80% activity at 50°C, 100% activity at 60°C, about 30% activity at 70°C | Geobacillus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 55 | 75 | the enzyme remains stable for up to 60 min at 55°C, after 60 min at 60 and 65°C the enzyme shows 75% activity, while after 20 min at 70 and 75°C the enzyme is inactive | Geobacillus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.133 | 15.38 | - |
amylopectin | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. | |
3.2.1.133 | 62.82 | - |
amylose | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. | |
3.2.1.133 | 225.6 | - |
gamma-cyclodextrin | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. | |
3.2.1.133 | 280.8 | - |
beta-cyclodextrin | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. | |
3.2.1.133 | 500 | - |
alpha-cyclodextrin | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 6 | - |
- |
Geobacillus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 5 | 9 | about 35% activity at pH 5.0, 100% activity at pH 6.0, about 85% activity at pH 7.0, about 60% activity at pH 8.0, about 40% activity at pH 9.0 | Geobacillus sp. |