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Literature summary extracted from

  • Rosa, L.; Ravanal, M.C.; Mardones, W.; Eyzaguirre, J.
    Characterization of a recombinant alpha-glucuronidase from Aspergillus fumigatus (2013), Fungal Biol., 117, 380-387.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.131 expressed in Pichia pastoris strain GS115 Aspergillus fumigatus
3.2.1.139 from strain A1163, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Pichia pastoris Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.139 extracellular
-
 Aspergillus fumigatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.131 91725
-
 2 * 91725, calculated from amino acid sequence Aspergillus fumigatus
3.2.1.131 100000
-
 x * 100000, SDS-PAGE Aspergillus fumigatus
3.2.1.139 91730
-
 recombinant mature enzyme, sequence calculation Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.131 Aspergillus fumigatus
-
-
-
3.2.1.139 Aspergillus fumigatus
-
-
-
3.2.1.139 Aspergillus fumigatus A1163
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.131 Ni-NTA column chromatography, and gel filtration Aspergillus fumigatus
3.2.1.139 recombinant His-tagged enzyme from Pichia pastoris by nickel affinity chromatography Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.139 additional information growth substrate is birchwood xylan Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.139 0.29
-
 recombinant His-tagged enzyme from Pichia pastoris, pH 5.0, 28°C Aspergillus fumigatus
3.2.1.139 1.09
-
 fungus native culture supernatant, pH 5.0, 28°C Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.131 birchwood xylan oligosaccharides + H2O the enzyme is active only in the presence of small oligosaccharides from birchwood xylan Aspergillus fumigatus ?
-
 ?
3.2.1.131 birchwood xylan oligosaccharides + H2O the enzyme is active only in the presence of small oligosaccharides from birchwood xylan Aspergillus fumigatus A1163 ?
-
 ?
3.2.1.131 additional information the enzyme binds strongly to cellulose without hydrolyzing it Aspergillus fumigatus ?
-
 ?
3.2.1.131 additional information the enzyme is inactive using birchwood xylan as substrate Aspergillus fumigatus ?
-
 ?
3.2.1.131 additional information the enzyme binds strongly to cellulose without hydrolyzing it Aspergillus fumigatus A1163 ?
-
 ?
3.2.1.131 additional information the enzyme is inactive using birchwood xylan as substrate Aspergillus fumigatus A1163 ?
-
 ?
3.2.1.139 additional information alpha-glucuronidases act on xylan side-chains and release methyl glucuronic acid residues. The enzyme is active on xylooligosaccharides generated from birchwood glucuronoxylan by a family 10 endoxylanase and on a mixture of aldouronic acids. No activity with birchwood glucuronoxylan Aspergillus fumigatus ?
-
 ?
3.2.1.139 additional information alpha-glucuronidases act on xylan side-chains and release methyl glucuronic acid residues. The enzyme is active on xylooligosaccharides generated from birchwood glucuronoxylan by a family 10 endoxylanase and on a mixture of aldouronic acids. No activity with birchwood glucuronoxylan Aspergillus fumigatus A1163 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.131 ? x * 100000, SDS-PAGE Aspergillus fumigatus
3.2.1.131 ? 2 * 91725, calculated from amino acid sequence Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.131 AguA
-
 Aspergillus fumigatus
3.2.1.131 alpha-glucuronidase
-
 Aspergillus fumigatus
3.2.1.139 AguA
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.131 37
-
-
 Aspergillus fumigatus
3.2.1.139 37
-
-
 Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.131 4.5 5
-
 Aspergillus fumigatus
3.2.1.139 4.5 5
-
 Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
3.2.1.139 evolution the enzyme is classified in family 67 of the glycosyl hydrolases Aspergillus fumigatus
3.2.1.139 metabolism the enzyme acts in synergy with endoxylanases and other xylanolytic enzymes in the biodegradation of xylan Aspergillus fumigatus
3.2.1.139 additional information the enzyme binds strongly to cellulose, thus it likely possesses a carbohydrate binding module Aspergillus fumigatus