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Literature summary extracted from

  • Maller, A.; da Silva, T.M.; Damasio, A.R.; Hirata, I.Y.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.
    Functional properties of a manganese-activated exo-polygalacturonase produced by a thermotolerant fungus Aspergillus niveus (2013), Folia Microbiol. (Praha), 58, 615-621.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.67 anion exchange resins stabilize the enzyme Aspergillus niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 additional information
-
 additional information 6.7 mg/ml with [(1->4)-alpha-D-galacturonide]n, pH 4.0, 50°C Aspergillus niveus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Aspergillus niveus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.67 Br- activates by 10% at 10 mg/ml Aspergillus niveus
3.2.1.67 F- activates by 17% at 10 mg/ml Aspergillus niveus
3.2.1.67 K+ activates by 17% at 10 mg/ml Aspergillus niveus
3.2.1.67 Mn2+ activates by 232% at 10 mg/ml Aspergillus niveus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 102600
-
 x * 102600, SDS-PAGE Aspergillus niveus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O Aspergillus niveus
-
 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O Aspergillus niveus RP05
-
 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Aspergillus niveus
-
 isolated from decomposing materials
-
3.2.1.67 Aspergillus niveus RP05
-
 isolated from decomposing materials
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.67 glycoprotein glycoprotein with 37.7% carbohydrate Aspergillus niveus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 native extracellular enzyme 4.4fold by anion exchange chromatography and affinity chromatography Aspergillus niveus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.67 additional information highest enzyme production at 40°C Aspergillus niveus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O
-
 Aspergillus niveus [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O polypectate Aspergillus niveus [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O
-
 Aspergillus niveus RP05 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O polypectate Aspergillus niveus RP05 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 ? x * 102600, SDS-PAGE Aspergillus niveus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 Exo-PG
-
 Aspergillus niveus
3.2.1.67 exo-polygalacturonase
-
 Aspergillus niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 50
-
-
 Aspergillus niveus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.67 393.3
-
 [(1->4)-alpha-D-galacturonide]n pH 4.0, 50°C Aspergillus niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 4 6.5
-
 Aspergillus niveus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 3 9 purified enzyme, 24 h, stable, mainly at pH 6.5, retaining more than 70% activity at pH 3.0 and 85% at pH 8.0 Aspergillus niveus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.67 Aspergillus niveus
-
-
 5.4

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.67 additional information
-
 additional information 58.7 mg/ml/s with [(1->4)-alpha-D-galacturonide]n, pH 4.0, 50°C Aspergillus niveus