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Literature summary extracted from

  • Uehara, R.; Tanaka, S.; Takano, K.; Koga, Y.; Kanaya, S.
    Requirement of insertion sequence IS1 for thermal adaptation of Pro-Tk-subtilisin from hyperthermophilic archaeon (2012), Extremophiles, 16, 841-851.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B57 expression of wid-type and mutant enzymes in Escherichia coli strain BL21(DE3) Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.B57 additional information generation of IS1-deletion mutants of S324A and S324C enzyme variants Thermococcus kodakarensis
3.4.21.B57 S324A site-directed mutagenesis, structure comparison of the mutant pro-enzyme with the wild-type pro-enzyme Thermococcus kodakarensis
3.4.21.B57 S324C site-directed mutagenesis, structure comparison of the mutant pro-enzyme with the wild-type pro-enzyme Thermococcus kodakarensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.B57 extracellular the enzyme is secreted into the external medium, probably as inactive pro-enzyme, with the assistance of the signal sequence Thermococcus kodakarensis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B57 additional information Thermococcus kodakarensis the propeptide is effectively degraded by the mature enzyme only at high temperatures, because it is too stable to be degraded at moderate temperatures ?
-
 ?
3.4.21.B57 pro-Tk-subtilisin + H2O Thermococcus kodakarensis autoactivation Tk-subtilisin + propeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B57 Thermococcus kodakarensis P58502
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B57 proteolytic modification prepro-Tk-subtilisin (Prepro-TKS), which consists of the signal sequence [Met (-24)-Ala(-1)], propeptide (Gly1-Leu69), and mature domain (Tk-subtilisin, Gly70-Gly398). Tk-subtilisin matures from Pro-Tk-subtilisin upon autoprocessing and degradation of propeptide. The pro-enzyme form contains the insertion sequence, IS1, at the N-terminus of the mature domain which is required not only for hyperstabilization of Pro-Tk-subtilisin but also for its rapid maturation, Most part of IS1 (Gly70-Gly78) is autocatalytically removed when Pro-TKS matures to Tk-subtilisin, structure and mechanism, overview Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B57 additional information the propeptide is effectively degraded by the mature enzyme only at high temperatures, because it is too stable to be degraded at moderate temperatures Thermococcus kodakarensis ?
-
 ?
3.4.21.B57 pro-Tk-subtilisin + H2O autoactivation Thermococcus kodakarensis Tk-subtilisin + propeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B57 Tk-subtilisin
-
 Thermococcus kodakarensis
3.4.21.B57 TKS
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B57 20
-
 assay at Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B57 9.5
-
 assay at Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
3.4.21.B57 additional information prepro-Tk-subtilisin (Prepro-TKS), which consists of the signal sequence [Met (-24)-Ala(-1)], propeptide (Tkpro, Gly1-Leu69), and mature domain (Tk-subtilisin, Gly70-Gly398). the pro-enzyme form contains the insertion sequence, IS1, at the N-terminus of the mature domain which is required not only for hyperstabilization of Pro-TKS but also for its rapid maturation Thermococcus kodakarensis