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Literature summary extracted from
- Production of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide (2014), Enzyme Microb. Technol., 64-65, 38-43.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | expressed in Escherichia coli BL21(DE3)pLyS cells | Thermus thermophilus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 53000 | - |
x * 53000, SDS-PAGE | Thermus thermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Thermus thermophilus | Q8GEB4 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | Ni-NTA column chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | stevioside + H2O | - |
Thermus thermophilus | rubusoside + D-glucose | i.e. 13-O-beta-glucosyl-19-O-beta-D-glucosyl-steviol, the optimum rubusoside synthesis yield is 86% at 200 g/l with 1200 units lactase | ? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 53000, SDS-PAGE | Thermus thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | beta-GLYPI | - |
Thermus thermophilus |
| 3.2.1.21 | lactase | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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