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Literature summary extracted from

  • Kaewmuangmoon, J.; Kilaso, M.; Leartsakulpanich, U.; Kimura, K.; Kimura, A.; Chanchao, C.
    Expression of a secretory alpha-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization (2013), BMC Biotechnol., 13, 16.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 gene AciHBGase II, DNA and amino acid sequence determination and analysis, recombinant expression of the C-terminally His-tagged secretory enzyme in Pichia pastoris strain GS115 Apis cerana indica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
additional information Michaelis-Menten kinetics Apis cerana indica
3.2.1.20 4.2
-
4-nitrophenyl alpha-D-glucoside recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 15.3
-
sucrose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 22
-
soluble starch recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 22.3
-
isomaltose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 25
-
maltotetraose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 26.7
-
maltotriose recombinant enzyme, pH 5.5, 37°C Apis cerana indica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 extracellular secretory enzyme Apis cerana indica
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Apis cerana indica J9SJ08 gene AciHBGase II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant extracellular His-tagged enzyme 1.45fold from Pichia pastoris strain GS115 culture supernatant by ultrafiltration, nickel affinity chromatography to homogeneity Apis cerana indica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 larva
-
Apis cerana indica
-
3.2.1.20 additional information highest transcript expression level of the isozyme is found in larvae and pupae, with lower levels in the eggs, no activity in foragers, comparison of expression patterns to isozyme I and III and to isozymes from Apis mellifera, overview Apis cerana indica
-
3.2.1.20 pupa
-
Apis cerana indica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 1.6
-
purified recombinant His-tagged enzyme, pH 5.5, 37°C, substrate 4-nitrophenyl alpha-D-glucoside Apis cerana indica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucoside + H2O low activity Apis cerana indica 4-nitrophenol + D-glucose
-
?
3.2.1.20 isomaltose + H2O low activity Apis cerana indica ?
-
?
3.2.1.20 maltose + H2O low activity Apis cerana indica ?
-
?
3.2.1.20 maltotetraose + H2O low activity Apis cerana indica ?
-
?
3.2.1.20 maltotriose + H2O low activity Apis cerana indica ?
-
?
3.2.1.20 additional information the enzyme shows a wide substrate specificity cleaving alpha-(1,1)-, alpha-(1,2)-, alpha-(1,4)- and alpha-(1,6)-glucosidic linkages, and rapidly hydrolyzing 4-nitrophenyl alpha-D-glucoside alpha-(1,6) and sucrose alpha-(1,2) linkages. Recombinant and native display positive cooperativity to sucrose, turanose alpha-(1,3), kojibiose alpha-(1,2) and soluble starch alpha-(1,4), the recombinant His-tagged enzyme hydrolyzes isomaltose and soluble starch but with different Km values Apis cerana indica ?
-
?
3.2.1.20 soluble starch + H2O low activity Apis cerana indica ?
-
?
3.2.1.20 sucrose + H2O highly preferred substrate Apis cerana indica ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-glucosidase II
-
Apis cerana indica
3.2.1.20 HBGase II
-
Apis cerana indica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 45
-
recombinant His-tagged enzyme Apis cerana indica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 40 55 recombinant His-tagged enzyme, over 80% of maximal activity in this range Apis cerana indica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 25 65 purified recombinant His-tagged enzyme, over 80% activity remaining Apis cerana indica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 1.89
-
4-nitrophenyl alpha-D-glucoside recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 2.84
-
maltotetraose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 4.6
-
soluble starch recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 5.2
-
isomaltose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 26.6
-
maltotriose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 845
-
sucrose recombinant enzyme, pH 5.5, 37°C Apis cerana indica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 3.5
-
recombinant His-tagged enzyme Apis cerana indica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 3.5 5 recombinant His-tagged enzyme, over 80% of maximal activity in this range Apis cerana indica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 4 8 purified recombinant His-tagged enzyme, over 80% activity remaining Apis cerana indica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.20 0.11
-
maltotetraose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 0.21
-
soluble starch recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 0.23
-
isomaltose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 0.45
-
4-nitrophenyl alpha-D-glucoside recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 1
-
maltotriose recombinant enzyme, pH 5.5, 37°C Apis cerana indica
3.2.1.20 55.4
-
sucrose recombinant enzyme, pH 5.5, 37°C Apis cerana indica