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Literature summary extracted from

  • Ma, S.; Tan, Y.L.; Yu, W.G.; Han, F.
    Cloning, expression and characterization of a new iota-carrageenase from marine bacterium, Cellulophaga sp. (2013), Biotechnol. Lett., 35, 1617-1622.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.157 gene cgiB_Ce, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3) Cellulophaga sp.
3.2.1.157 gene cgiB_Ce, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Cellulophaga sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.157 Al3+ 56% inhibition at 1 mM Cellulophaga sp.
3.2.1.157 Cu2+ 87% inhibition at 1 mM Cellulophaga sp.
3.2.1.157 EDTA 90% inhibition at 1 mM Cellulophaga sp.
3.2.1.157 Fe3+ 11% inhibition at 1 mM Cellulophaga sp.
3.2.1.157 SDS 97% inhibition at 1 mM Cellulophaga sp.
3.2.1.157 Zn2+ 49% inhibition at 1 mM Cellulophaga sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.157 Ba2+ 118% activation at 1 mM Cellulophaga sp.
3.2.1.157 Ca2+ 122% activation at 1 mM Cellulophaga sp.
3.2.1.157 Fe2+ 174% activation at 1 mM Cellulophaga sp.
3.2.1.157 K+ 134% activation at 10 mM Cellulophaga sp.
3.2.1.157 Li+ 114% activation at 1 mM Cellulophaga sp.
3.2.1.157 Mg2+ 149% activation at 1 mM Cellulophaga sp.
3.2.1.157 Mn2+ 173% activation at 1 mM Cellulophaga sp.
3.2.1.157 Na+ 166% activation at 500 mM Cellulophaga sp.
3.2.1.157 NaCl although its activity is enhanced by NaCl, the enzyme is active in the absence of NaCl Cellulophaga sp.
3.2.1.157 Ni2+ 128% activation at 1 mM Cellulophaga sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.157 48071
-
 x * 48071, sequence calculation Cellulophaga sp.
3.2.1.157 48071
-
 x * 48071, sequence calculation, x * 54000, recombinant His-tagged enzyme, SDS-PAGE Cellulophaga sp.
3.2.1.157 54000
-
 x * 48071, sequence calculation, x * 54000, recombinant His-tagged enzyme, SDS-PAGE Cellulophaga sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.157 iota-carrageenan + H2O Cellulophaga sp.
-
 neo-iota-carratetraose
-
 ?
3.2.1.157 iota-carrageenan + H2O Cellulophaga sp. yielding neo-iota-carratetraose as the main product in the absence of NaCl neo-iota-carratetraose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.157 Cellulophaga sp. R9UQG9 gene cgiB_Ce
-
3.2.1.157 Cellulophaga sp. R9UQG9 isolated from the red alga Grateloupia livida collected from Qingdao coastal waters of China Yellow Sea, gene cgiB_Ce
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.157 recombinant enzyme from Escherichia coli strain BL21(DE3) culture supernatant by affinity chromatography Cellulophaga sp.
3.2.1.157 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Cellulophaga sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.157 1870
-
 pH 6.5, 45°C, with iota-carrageenan Cellulophaga sp.
3.2.1.157 1870
-
 purified enzyme, pH 6.5, 45°C Cellulophaga sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.157 iota-carrageenan + H2O
-
 Cellulophaga sp. neo-iota-carratetraose
-
 ?
3.2.1.157 iota-carrageenan + H2O yielding neo-iota-carratetraose as the main product in the absence of NaCl Cellulophaga sp. neo-iota-carratetraose
-
 ?
3.2.1.157 iota-carrageenan + H2O the endo-type iota-carrageenase hydrolyzes beta-1,4-linkages of iota-carrageenan Cellulophaga sp. neo-iota-carratetraose neo-carratetraose is the main product with more than 80% of the total product ?
3.2.1.157 iota-carrageenan + H2O the endo-type iota-carrageenase hydrolyzes beta-1,4-linkages of iota-carrageenan yielding neo-iota-carratetraose as the main product in the absence of NaCl, mass and NMR spectrometric product analysis, overview Cellulophaga sp. neo-iota-carratetraose
-
 ?
3.2.1.157 additional information no activity towards jota-carrageenan, kappa-carrageenan or agarose Cellulophaga sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.157 ? x * 48071, sequence calculation Cellulophaga sp.
3.2.1.157 ? x * 48071, sequence calculation, x * 54000, recombinant His-tagged enzyme, SDS-PAGE Cellulophaga sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.157 CgiB_Ce
-
 Cellulophaga sp.
3.2.1.157 endo-type iota-carrageenase
-
 Cellulophaga sp.
3.2.1.157 iota-carrageenase
-
 Cellulophaga sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.157 45
-
-
 Cellulophaga sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.157 15 65 activity range, overview Cellulophaga sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.157 40
-
 stable up to Cellulophaga sp.
3.2.1.157 40
-
 purified recombinant His-tagged enzyme, pH 6.5, 1 h, 80% activity remaining Cellulophaga sp.
3.2.1.157 40
-
 purified recombinant His-tagged enzyme, pH 6.5, 1 h, inactivation Cellulophaga sp.
3.2.1.157 50
-
 purified recombinant His-tagged enzyme, pH 6.5, 1 h, 60% activity remaining Cellulophaga sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.157 6.5
-
-
 Cellulophaga sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.157 5 11 activity range, overview Cellulophaga sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.157 6 9 purified recombinant His-tagged enzyme, 45°C, 24 h, stable Cellulophaga sp.
3.2.1.157 6 9.6 stable at Cellulophaga sp.
3.2.1.157 10
-
 purified recombinant His-tagged enzyme, 45°C, 24 h, 60% activity remaining Cellulophaga sp.
3.2.1.157 11
-
 purified recombinant His-tagged enzyme, 45°C, 24 h, inactivation Cellulophaga sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.157 Cellulophaga sp. sequence calculation
-
 4.79

General Information

EC Number General Information Comment Organism
3.2.1.157 evolution the enzyme belongs to glycohydrolase family 82, GH82, forming a deeply branched cluster in the phylogenetic tree, along with Celly_2571 and CgiA3, distinct from other iota-carrageenases Cellulophaga sp.
3.2.1.157 evolution the enzyme belongs to the glycosyl hydrolase family 82, GH82 Cellulophaga sp.