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Literature summary extracted from

  • Lee, A.R.; Bak, H.J.; Kim, N.Y.; Kim, M.S.; Go, H.J.; Han, J.W.; Ahn, S.J.; Park, N.G.; Chung, J.K.; Lee, H.H.
    Cloning, heterologous expression, and enzymatic characterization of cathepsin L from starfish (Asterina pectinifera) (2012), Biosci. Biotechnol. Biochem., 76, 2342-2346.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.15 expressed in Escherichia coli BL21(DE3) cells Patiria pectinifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 antipain
-
 Patiria pectinifera
3.4.22.15 chymostatin
-
 Patiria pectinifera
3.4.22.15 Co2+ 58.88% residual activity at 5 mM Patiria pectinifera
3.4.22.15 E-64
-
 Patiria pectinifera
3.4.22.15 Hg2+ 19.61% residual activity at 5 mM Patiria pectinifera
3.4.22.15 leupeptin
-
 Patiria pectinifera
3.4.22.15 additional information not inhibited by N-ethylmaleimide, phenylmethylsulfonyl fluoride, antiprotinin, EDTA, EGTA, 1,10-phenanthroline, and pepstatin A Patiria pectinifera
3.4.22.15 SDS 6.53% residual activity at 0.05% (w/v) Patiria pectinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 61000
-
 x * 61000, pro-enzyme fused to GST, SDS-PAGE Patiria pectinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.15 Gelatin + H2O Patiria pectinifera
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Patiria pectinifera I6QMK7 synonym Asterina pectinifera, starfisch
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15 glutathione Sepharose column chromatography, and gel filtration Patiria pectinifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.15 liver
-
 Patiria pectinifera
-
3.4.22.15 muscle
-
 Patiria pectinifera
-
3.4.22.15 stomach
-
 Patiria pectinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Patiria pectinifera benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Patiria pectinifera benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O most efficient substrate Patiria pectinifera benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Patiria pectinifera benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 Gelatin + H2O
-
 Patiria pectinifera ?
-
 ?
3.4.22.15 additional information the recombinant enzyme is inefficient in hydrolyzing azocasein, fibrinogen, and bovine serum albumin Patiria pectinifera ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ? x * 61000, pro-enzyme fused to GST, SDS-PAGE Patiria pectinifera

Synonyms

EC Number Synonyms Comment Organism
3.4.22.15 CtL
-
 Patiria pectinifera

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.15 20 50 the enzyme is stable between 20 and 50°C, but activity drops above 60°C Patiria pectinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 8
-
-
 Patiria pectinifera