Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schalk, A.M.; Lavie, A.
    Structural and kinetic characterization of guinea pig L-asparaginase type III (2014), Biochemistry, 53, 2318-2328.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 glycine glycine stimulates self-cleavage in a dose-dependent manner Cavia porcellus

Application

EC Number Application Comment Organism
3.4.19.5 medicine isoform ASRGL1 is not the asparaginase responsible for the antitumor effects elicited by treatment with guinea pig serum Cavia porcellus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.5 expression in Escherichia coli Cavia porcellus
3.5.1.1 expressed in Escherichia coli BL21(DE3) cells Cavia porcellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.19.5 uncleaved (inactive) as well as the cleaved (active), aspartate-bound structures. Cleavage does not greatly alter the overall structure of the enzyme with the exception of the conformational change in the conserved HGG loop that coincides with cleavage Cavia porcellus
3.5.1.1 hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M MES at pH 6.0, and 13-15% (w/v) PEG 3350 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.5 0.41
-
 beta-Asp-Phe pH 7.5, 37°C Cavia porcellus
3.5.1.1 0.41
-
 beta-L-Asp-L-Phe at pH 7.5 and 37°C Cavia porcellus
3.5.1.1 2.24
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.5 15000
-
 beta-subunit, SDS-PAGE Cavia porcellus
3.4.19.5 20000
-
 alpha-subunit, SDS-PAGE Cavia porcellus
3.4.19.5 35000
-
 inactive precursor, SDS-PAGE Cavia porcellus
3.5.1.1 15000
-
 1 * 20000 + 1 * 15000, active, cleaved enzyme Cavia porcellus
3.5.1.1 20000
-
 1 * 20000 + 1 * 15000, active, cleaved enzyme Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Cavia porcellus
-
 L-aspartate + NH3
-
 ?
3.5.1.1 additional information Cavia porcellus the enzyme requires autocleavage to become active ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.5 Cavia porcellus
-
-
-
3.5.1.1 Cavia porcellus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.19.5 proteolytic modification inactive precursor undergoes a self-activation process, converting it from an uncleaved precursor of 35000 Da into alpha- and beta-subunits of 20000 and 15000 Da, respectively. Glycine stimulates cleavage in a dose-dependent manner Cavia porcellus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 HisTrap HP Ni Sepharose column chromatography and Superdex 200 gel filtration Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.5 beta-Asp-Phe + H2O
-
 Cavia porcellus L-Asp + L-Phe
-
 ?
3.4.19.5 additional information enzyme additionally acts as an asparaginase, EC 3.5.1.1 Cavia porcellus ?
-
 ?
3.5.1.1 beta-L-Asp-L-Phe + H2O
-
 Cavia porcellus ?
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Cavia porcellus L-aspartate + NH3
-
 ?
3.5.1.1 additional information the enzyme requires autocleavage to become active Cavia porcellus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 heterodimer 1 * 20000 + 1 * 15000, active, cleaved enzyme Cavia porcellus

Synonyms

EC Number Synonyms Comment Organism
3.4.19.5 ASNase3
-
 Cavia porcellus
3.4.19.5 ASRGL1
-
 Cavia porcellus
3.5.1.1 ASNase3
-
 Cavia porcellus
3.5.1.1 L-asparaginase type III
-
 Cavia porcellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.19.5 1.98
-
 beta-Asp-Phe pH 7.5, 37°C Cavia porcellus
3.5.1.1 1.98
-
 beta-L-Asp-L-Phe at pH 7.5 and 37°C Cavia porcellus
3.5.1.1 3.95
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus

General Information

EC Number General Information Comment Organism
3.5.1.1 additional information ASNase3 is not the enzyme responsible for the antitumor effects of guinea pig serum Cavia porcellus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.19.5 4.83
-
 beta-Asp-Phe pH 7.5, 37°C Cavia porcellus
3.5.1.1 1.76
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus
3.5.1.1 4.83
-
 beta-L-Asp-L-Phe at pH 7.5 and 37°C Cavia porcellus