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Literature summary extracted from

  • Yano, T.; Rahimian, M.; Aneja, K.K.; Schechter, N.M.; Rubin, H.; Scott, C.P.
    Mycobacterium tuberculosis type II NADH-menaquinone oxidoreductase catalyzes electron transfer through a two-site ping-pong mechanism and has two quinone-binding sites (2014), Biochemistry, 53, 1179-1190.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.2 expression in Mycobacterium smegmatis Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.2 0.02
-
 ubiquinone-2 pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
7.1.1.2 0.1
-
 menaquinone pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
7.1.1.2 0.12
-
 NADH cosubstrate ubiquinone-2, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
7.1.1.2 0.14
-
 NADH cosubstrate ubiquinone-0, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
7.1.1.2 0.23
-
 NADH cosubstrate menaquinone, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
7.1.1.2 0.35
-
 ubiquinone-0 pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 additional information enzyme catalyzes the transfer electrons from NADH to quinone substrates by a nonclassical, two-site pingpong kinetic mechanism whereby substrate quinones bind to a site that is distinct from the NADH-binding site. Presence of two binding sites for quinone ligands, one favoring the reduced form and the other favoring the oxidized form Mycobacterium tuberculosis ?
-
 ?
7.1.1.2 NADH + H+ + menaquinone
-
 Mycobacterium tuberculosis NAD+ + menaquinol
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-0
-
 Mycobacterium tuberculosis NAD+ + ubiquinol-0
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-1
-
 Mycobacterium tuberculosis NAD+ + ubiquinol-1
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-2
-
 Mycobacterium tuberculosis NAD+ + ubiquinol-2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 NDH-2
-
 Mycobacterium tuberculosis
7.1.1.2 type II NADH-quinone oxidoreductase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.2 423
-
 menaquinone pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
7.1.1.2 480
-
 ubiquinone-0 pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
7.1.1.2 640
-
 ubiquinone-2 pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis