EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.1 | overexpressed as His6-tagged protein in Escherichia coli cells. The production of the protein corresponding to the gene GOT1L1 encounters several problems: upon induction, multiple overexpressed protein species are detected in the cell lysate, and none of them could be purified by metal-affinity chromatography | Homo sapiens |
2.6.1.2 | overexpressed as His6-tagged protein in Escherichia coli cells | Homo sapiens |
2.6.1.4 | overexpressed as His6-tagged protein in Escherichia coli cells | Homo sapiens |
2.6.1.52 | overexpressed as His6-tagged protein in Escherichia coli cells | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.2 | 0.75 | - |
glyoxylate | pH 7.4, 37°C, cosubstrate: L-glutamate | Homo sapiens | |
2.6.1.2 | 0.81 | - |
glyoxylate | pH 7.4, 37°C, cosubstrate: L-alanine | Homo sapiens | |
2.6.1.4 | 0.75 | - |
glyoxylate | pH 7.4, 37°C, cosubstrate: L-glutamate | Homo sapiens | |
2.6.1.4 | 0.81 | - |
glyoxylate | pH 7.4, 37°C, cosubstrate: L-alanine | Homo sapiens | |
2.6.1.4 | 5.5 | - |
glyoxylate | pH 7.4, 37°C, cosubstrate: L-glutamate | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.6.1.1 | cytosol | - |
Homo sapiens | 5829 | - |
2.6.1.2 | cytosol | - |
Homo sapiens | 5829 | - |
2.6.1.4 | cytosol | - |
Homo sapiens | 5829 | - |
2.6.1.52 | cytosol | - |
Homo sapiens | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.2 | 57000 | - |
recombinant enzyme | Homo sapiens |
2.6.1.4 | 57000 | - |
recombinant enzyme | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.1 | Homo sapiens | Q8NHS2 | - |
- |
2.6.1.2 | Homo sapiens | P24298 | - |
- |
2.6.1.4 | Homo sapiens | P24298 | - |
- |
2.6.1.4 | Homo sapiens | Q9Y617 | - |
- |
2.6.1.52 | Homo sapiens | Q9Y617 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.2 | - |
Homo sapiens |
2.6.1.4 | - |
Homo sapiens |
2.6.1.52 | - |
Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.2 | 2.1 | - |
pH 7.4, 25°C, L-alanine (10 mM) + glyoxylate | Homo sapiens |
2.6.1.2 | 2.5 | - |
pH 7.4, 25°C, L-glutamate (10 mM) + glyoxylate | Homo sapiens |
2.6.1.4 | 1.5 | - |
pH 7.4, 25°C, L-glutamate (1 mM) + glyoxylate | Homo sapiens |
2.6.1.4 | 2.1 | - |
pH 7.4, 25°C, L-alanine (10 mM) + glyoxylate | Homo sapiens |
2.6.1.4 | 2.5 | - |
pH 7.4, 25°C, L-glutamate (10 mM) + glyoxylate | Homo sapiens |
2.6.1.4 | 87 | - |
pH 7.5, 25°C, L-alanine + 2-oxoglutarate | Homo sapiens |
2.6.1.52 | 12 | - |
pH and temperature not specified in the publication | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.1 | L-aspartate + 2-oxoglutarate | - |
Homo sapiens | oxaloacetate + L-glutamate | - |
? | |
2.6.1.2 | L-alanine + glyoxylate | L-glutamate and L-alanine are by far the best amino-group donors | Homo sapiens | pyruvate + glycine | - |
? | |
2.6.1.2 | L-glutamate + glyoxylate | L-glutamate and L-alanine are by far the best amino-group donors | Homo sapiens | 2-oxoglutarate + glycine | - |
? | |
2.6.1.4 | L-alanine + 2-oxoglutarate | - |
Homo sapiens | pyruvate + L-glutamate | - |
? | |
2.6.1.4 | L-alanine + glyoxylate | - |
Homo sapiens | pyruvate + glycine | - |
? | |
2.6.1.4 | L-alanine + glyoxylate | L-glutamate and L-alanine are by far the best amino-group donors for glyoxylate | Homo sapiens | pyruvate + glycine | - |
? | |
2.6.1.4 | L-asparagine + glyoxylate | - |
Homo sapiens | 4-amino-2,4-dioxobutanoate + glycine | - |
? | |
2.6.1.4 | L-glutamate + glyoxylate | L-Glu is the preferred amino-group donor both at 25°C and at 37°C | Homo sapiens | 2-oxoglutarate + glycine | - |
? | |
2.6.1.4 | L-glutamate + glyoxylate | L-glutamate and L-alanine are by far the best amino-group donors for glyoxylate | Homo sapiens | 2-oxoglutarate + glycine | - |
? | |
2.6.1.4 | L-homocysteate + glyoxylate | - |
Homo sapiens | ? + glycine | - |
? | |
2.6.1.4 | L-serine + glyoxylate | - |
Homo sapiens | 3-hydroxy-2-oxopropanoate + glycine | - |
? | |
2.6.1.4 | additional information | phosphoserine is not an efficient amino-group donor | Homo sapiens | ? | - |
? | |
2.6.1.52 | 3-phosphonooxypyruvate + L-glutamate | - |
Homo sapiens | O-phospho-L-serine + 2-oxoglutarate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.1 | GOT1L1 | - |
Homo sapiens |
2.6.1.2 | GPT | - |
Homo sapiens |
2.6.1.4 | GPT | - |
Homo sapiens |
2.6.1.4 | PSAT1 | - |
Homo sapiens |
2.6.1.52 | PSAT1 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 25 | - |
assay at, coupled assay with malate dehydrogenase | Homo sapiens |
2.6.1.2 | 25 | - |
assay at | Homo sapiens |
2.6.1.2 | 37 | - |
assay at | Homo sapiens |
2.6.1.4 | 25 | - |
assay at | Homo sapiens |
2.6.1.4 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 7.5 | - |
assay at, coupled assay with malate dehydrogenase | Homo sapiens |
2.6.1.2 | 7.4 | - |
assay at | Homo sapiens |
2.6.1.4 | 7.4 | - |
assay at | Homo sapiens |
2.6.1.52 | 7.4 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.2 | pyridoxal 5'-phosphate | - |
Homo sapiens | |
2.6.1.4 | pyridoxal 5'-phosphate | - |
Homo sapiens | |
2.6.1.52 | pyridoxal 5'-phosphate | - |
Homo sapiens |