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Literature summary extracted from

  • Donini, S.; Ferrari, M.; Fedeli, C.; Faini, M.; Lamberto, I.; Marletta, A.S.; Mellini, L.; Panini, M.; Percudani, R.; Pollegioni, L.; Caldinelli, L.; Petrucco, S.; Peracchi, A.
    Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism (2009), Biochem. J., 422, 265-272.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.1 overexpressed as His6-tagged protein in Escherichia coli cells. The production of the protein corresponding to the gene GOT1L1 encounters several problems: upon induction, multiple overexpressed protein species are detected in the cell lysate, and none of them could be purified by metal-affinity chromatography Homo sapiens
2.6.1.2 overexpressed as His6-tagged protein in Escherichia coli cells Homo sapiens
2.6.1.4 overexpressed as His6-tagged protein in Escherichia coli cells Homo sapiens
2.6.1.52 overexpressed as His6-tagged protein in Escherichia coli cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.75
-
glyoxylate pH 7.4, 37°C, cosubstrate: L-glutamate Homo sapiens
2.6.1.2 0.81
-
glyoxylate pH 7.4, 37°C, cosubstrate: L-alanine Homo sapiens
2.6.1.4 0.75
-
glyoxylate pH 7.4, 37°C, cosubstrate: L-glutamate Homo sapiens
2.6.1.4 0.81
-
glyoxylate pH 7.4, 37°C, cosubstrate: L-alanine Homo sapiens
2.6.1.4 5.5
-
glyoxylate pH 7.4, 37°C, cosubstrate: L-glutamate Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.1 cytosol
-
Homo sapiens 5829
-
2.6.1.2 cytosol
-
Homo sapiens 5829
-
2.6.1.4 cytosol
-
Homo sapiens 5829
-
2.6.1.52 cytosol
-
Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 57000
-
recombinant enzyme Homo sapiens
2.6.1.4 57000
-
recombinant enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Homo sapiens Q8NHS2
-
-
2.6.1.2 Homo sapiens P24298
-
-
2.6.1.4 Homo sapiens P24298
-
-
2.6.1.4 Homo sapiens Q9Y617
-
-
2.6.1.52 Homo sapiens Q9Y617
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2
-
Homo sapiens
2.6.1.4
-
Homo sapiens
2.6.1.52
-
Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 2.1
-
pH 7.4, 25°C, L-alanine (10 mM) + glyoxylate Homo sapiens
2.6.1.2 2.5
-
pH 7.4, 25°C, L-glutamate (10 mM) + glyoxylate Homo sapiens
2.6.1.4 1.5
-
pH 7.4, 25°C, L-glutamate (1 mM) + glyoxylate Homo sapiens
2.6.1.4 2.1
-
pH 7.4, 25°C, L-alanine (10 mM) + glyoxylate Homo sapiens
2.6.1.4 2.5
-
pH 7.4, 25°C, L-glutamate (10 mM) + glyoxylate Homo sapiens
2.6.1.4 87
-
pH 7.5, 25°C, L-alanine + 2-oxoglutarate Homo sapiens
2.6.1.52 12
-
pH and temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
Homo sapiens oxaloacetate + L-glutamate
-
?
2.6.1.2 L-alanine + glyoxylate L-glutamate and L-alanine are by far the best amino-group donors Homo sapiens pyruvate + glycine
-
?
2.6.1.2 L-glutamate + glyoxylate L-glutamate and L-alanine are by far the best amino-group donors Homo sapiens 2-oxoglutarate + glycine
-
?
2.6.1.4 L-alanine + 2-oxoglutarate
-
Homo sapiens pyruvate + L-glutamate
-
?
2.6.1.4 L-alanine + glyoxylate
-
Homo sapiens pyruvate + glycine
-
?
2.6.1.4 L-alanine + glyoxylate L-glutamate and L-alanine are by far the best amino-group donors for glyoxylate Homo sapiens pyruvate + glycine
-
?
2.6.1.4 L-asparagine + glyoxylate
-
Homo sapiens 4-amino-2,4-dioxobutanoate + glycine
-
?
2.6.1.4 L-glutamate + glyoxylate L-Glu is the preferred amino-group donor both at 25°C and at 37°C Homo sapiens 2-oxoglutarate + glycine
-
?
2.6.1.4 L-glutamate + glyoxylate L-glutamate and L-alanine are by far the best amino-group donors for glyoxylate Homo sapiens 2-oxoglutarate + glycine
-
?
2.6.1.4 L-homocysteate + glyoxylate
-
Homo sapiens ? + glycine
-
?
2.6.1.4 L-serine + glyoxylate
-
Homo sapiens 3-hydroxy-2-oxopropanoate + glycine
-
?
2.6.1.4 additional information phosphoserine is not an efficient amino-group donor Homo sapiens ?
-
?
2.6.1.52 3-phosphonooxypyruvate + L-glutamate
-
Homo sapiens O-phospho-L-serine + 2-oxoglutarate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 GOT1L1
-
Homo sapiens
2.6.1.2 GPT
-
Homo sapiens
2.6.1.4 GPT
-
Homo sapiens
2.6.1.4 PSAT1
-
Homo sapiens
2.6.1.52 PSAT1
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.1 25
-
assay at, coupled assay with malate dehydrogenase Homo sapiens
2.6.1.2 25
-
assay at Homo sapiens
2.6.1.2 37
-
assay at Homo sapiens
2.6.1.4 25
-
assay at Homo sapiens
2.6.1.4 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 7.5
-
assay at, coupled assay with malate dehydrogenase Homo sapiens
2.6.1.2 7.4
-
assay at Homo sapiens
2.6.1.4 7.4
-
assay at Homo sapiens
2.6.1.52 7.4
-
assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 pyridoxal 5'-phosphate
-
Homo sapiens
2.6.1.4 pyridoxal 5'-phosphate
-
Homo sapiens
2.6.1.52 pyridoxal 5'-phosphate
-
Homo sapiens