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Literature summary extracted from

  • Hummel, W.; Schütte, H.; Kula, M.-R.
    D-2-Hydroxyisocaproate dehydrogenase from Lactobacillus casei (1985), Appl. Microbiol. Biotechnol., 21, 7-15.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.345 1,10-phenanthroline 10 mM, decrease of 13% in the enzymatic activity Lacticaseibacillus paracasei
1.1.1.345 Hg2+ 0.1 mM, complete inhibition Lacticaseibacillus paracasei
1.1.1.345 additional information the addition of citrate and EDTA (up to 10 mM) has no effect on the enzymatic activity. The addition of of iodoacetamide, KCN, 2-mercaptoethanol, dithiothreitol, and reduced glutathione in concentrations up to 20 mM has no effect Lacticaseibacillus paracasei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.345 0.01
-
 NADH pH 7.0, 25°C Lacticaseibacillus paracasei
1.1.1.345 0.06
-
 4-methyl-2-oxopentanoate pH 7.0, 25°C Lacticaseibacillus paracasei
1.1.1.345 0.11
-
 2-oxopentanoate pH 7.0, 25°C Lacticaseibacillus paracasei
1.1.1.345 0.11
-
 2-Oxohexanoate pH 7.0, 25°C Lacticaseibacillus paracasei
1.1.1.345 0.15
-
 phenylpyruvate pH 7.0, 25°C Lacticaseibacillus paracasei
1.1.1.345 0.5
-
 NAD+ pH 7.0, 25°C Lacticaseibacillus paracasei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.345 additional information no influence on the enzymatic activity can be detected with Mg2+ and Ca2+ and only very weak effects with Cd2+, Co2+ , and Mn2+ Lacticaseibacillus paracasei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.345 38000
-
 2 * 38000, SDS-PAGE Lacticaseibacillus paracasei
1.1.1.345 74000
-
 gel filtration Lacticaseibacillus paracasei

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.345 Lacticaseibacillus paracasei P17584
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.345
-
 Lacticaseibacillus paracasei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.345 2-oxohexanoate + NADH + H+ i.e. 2-oxocaproate Lacticaseibacillus paracasei 2-hydroxyhexanoate + NAD+
-
 r
1.1.1.345 2-oxopentanoate + NADH + H+ i.e. 2-oxovalerate Lacticaseibacillus paracasei 2-hydroxypentanoate + NAD+
-
 r
1.1.1.345 4-methyl-2-oxopentanoate + NADH + H+ i.e. 2-oxoisocaproate Lacticaseibacillus paracasei (R)-2-hydroxy-4-methylpentanoate + NAD+ i.e. (R)-2-hydroxyisocaproate r
1.1.1.345 phenylpyruvate + NADH + H+
-
 Lacticaseibacillus paracasei phenyllactate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.345 dimer 2 * 38000, SDS-PAGE Lacticaseibacillus paracasei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.345 D-2-hydroxyisocaproate dehydrogenase
-
 Lacticaseibacillus paracasei
1.1.1.345 HdhD
-
 Lacticaseibacillus paracasei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.345 25
-
 assay at Lacticaseibacillus paracasei
1.1.1.345 50
-
-
 Lacticaseibacillus paracasei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.345 45
-
 60 min, 95% of the activity can be recovered Lacticaseibacillus paracasei
1.1.1.345 50
-
 at temperatures above 50°C the stability of the enzyme decreases drastically Lacticaseibacillus paracasei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.345 5.5 7 reduction of 4-methyl-2-oxopentanoate Lacticaseibacillus paracasei
1.1.1.345 7
-
 assay at Lacticaseibacillus paracasei
1.1.1.345 8 9 oxidation of (R)-2-hydroxy-4-methylpentanoate Lacticaseibacillus paracasei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.345 3.3 9.7 6 weeks, more than 80% of the activity is recovered Lacticaseibacillus paracasei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.345 NAD+ with NADP+ no reaction was observed in concentrations up to 4.5 mM Lacticaseibacillus paracasei
1.1.1.345 NADH rhe enzyme requires NADH as a coenzyme for the hydrogenation reaction, NADPH does not support the reaction in concentrations up to 0.1 mM Lacticaseibacillus paracasei