EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.81 | expressed in Escherichia coli | Bacillus thuringiensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.81 | 0.5 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 0.86 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 3.81 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 8.84 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.81 | Al3+ | strongly inhibits enzymatic activity | Bacillus thuringiensis | |
3.1.1.81 | Cu2+ | abolishes enzymatic activity | Bacillus thuringiensis | |
3.1.1.81 | Fe2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
3.1.1.81 | K+ | weakly inhibits eznymatic activity | Bacillus thuringiensis | |
3.1.1.81 | Mg2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
3.1.1.81 | Mn2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
3.1.1.81 | Pb2+ | inhibits enzymatic activity | Bacillus thuringiensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | 28000 | - |
SDS-PAGE | Bacillus thuringiensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.81 | Bacillus thuringiensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.81 | using Ni-NTA chromatography | Bacillus thuringiensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.81 | N-butanoyl-DL-homoserine lactone + H2O | relative activity: 190% | Bacillus thuringiensis | N-butanoyl-DL-homoserine | - |
? | |
3.1.1.81 | N-heptanoyl-DL-homoserine lactone + H2O | relative activity: 73% | Bacillus thuringiensis | N-heptanoyl-DL-homoserine | - |
? | |
3.1.1.81 | N-hexanoyl-DL-homoserine lactone + H2O | relative activity: 100% | Bacillus thuringiensis | N-hexanoyl-DL-homoserine | - |
? | |
3.1.1.81 | N-octanoyl-DL-homoserine lactone + H2O | relative activity: 457% | Bacillus thuringiensis | N-octanoyl-DL-homoserine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.81 | AHL | - |
Bacillus thuringiensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | 30 | - |
- |
Bacillus thuringiensis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | 30 | 70 | enzyme shows reduced activity at 70°C | Bacillus thuringiensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | 30 | 70 | - |
Bacillus thuringiensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.81 | 0.00549 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 0.0102 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 0.0235 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 0.0446 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | 8 | - |
- |
Bacillus thuringiensis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | 6 | 9 | reduced activity at pH 6 | Bacillus thuringiensis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.81 | 0.00266 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 0.0109 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 0.0117 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.1.1.81 | 0.0119 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis |