EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.142 | DTT | - |
Hordeum vulgare |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.142 | recombinant expression in Pichia pastoris and Escherichia coli | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.142 | additional information | - |
additional information | Michaelis-Menten kinetics | Hordeum vulgare | |
3.2.1.142 | 0.135 | - |
2-chloro-4-nitrophenyl maltotriosyl-alpha(1->6)-maltotriosyl-beta-D-glucose | pH 5.3, 45°C, barley malt extract | Hordeum vulgare | |
3.2.1.142 | 0.154 | - |
2-chloro-4-nitrophenyl maltotriosyl-alpha(1->6)-maltotriosyl-beta-D-glucose | pH 5.3, 45°C, recombinant enzyme | Hordeum vulgare |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.142 | Ca2+ | required | Hordeum vulgare |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.142 | Hordeum vulgare | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.142 | 2-chloro-4-nitrophenyl maltotriosyl-alpha(1->6)-maltotriosyl-beta-D-glucose + H2O | - |
Hordeum vulgare | 2-chloro-4-nitrophenyl maltotriose + maltotriosyl-beta-D-glucose | - |
? | |
3.2.1.142 | additional information | no activity with glycogen | Hordeum vulgare | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.142 | 45 | - |
assay at | Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.142 | 5.3 | - |
assay at | Hordeum vulgare |