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Literature summary extracted from
- Structure of the starch-debranching enzyme barley limit dextrinase reveals homology of the N-terminal domain to CBM21 (2012), Acta Crystallogr. Sect. F, 68, 1008-1012.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.142 | recombinant epression in Pichia pastoris | Hordeum vulgare |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.142 | purified free enzyme with a glycerol molecule in the active site or purified enzyme in complex with competitive inhibitors alpha- and beta-cyclodextrin, hanging drop vapour diffusion method, streak-seeding, mixing of 10 mg/mlprotein in 50 mM MES buffer pH 6.6, 250 mM NaCl, 0.5 mM CaCl2, 0.67mM maltotriose, with a reservoir solution consisting of 30% w/v PEG 3350, 5% glycerol, 0.3 M NaI, cysteine is added to the crystallization drops to a final concentration of 5-7 mM, one week, 20°C, X-ray diffraction structure determination and analysis at 1.9-2.5 A resolution, molecular replacement and modelling | Hordeum vulgare |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.142 | alpha-cyclodextrin | competitive inhibitor | Hordeum vulgare |  |
| 3.2.1.142 | beta-cyclodextrin | competitive inhibitor | Hordeum vulgare | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.142 | limit dextrin + H2O | Hordeum vulgare | the enzyme hydrolyses alpha-1,6-glucosidic linkages in limit dextrins derived from amylopectin | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.142 | Hordeum vulgare | O48541 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.142 | limit dextrin + H2O | the enzyme hydrolyses alpha-1,6-glucosidic linkages in limit dextrins derived from amylopectin | Hordeum vulgare | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.142 | starch-debranching enzyme | - |
Hordeum vulgare |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.142 | evolution | the debranching enzyme belongs to the glycoside hydrolase family 13 subfamily 13, GH13_13 | Hordeum vulgare |
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Release 2023.1 (January 2023)
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