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Literature summary extracted from

  • Xu, Y.; Li, X.; Li, R.; Li, S.; Ni, H.; Wang, H.; Xu, H.; Zhou, W.; Saris, P.E.; Yang, W.; Qiao, M.; Rao, Z.
    Structure of the nisin leader peptidase NisP revealing a C-terminal autocleavage activity (2014), Acta Crystallogr. Sect. D, 70, 1499-1505.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.46 expressed in Lactococcus lactis strain LAC71 and in Escherichia coli BL21 (DE3) cells Lactococcus lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.46 hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 24-32% (v/v) 1,4-dioxane Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.46 H306A inactive Lactococcus lactis
3.4.22.46 R647P/S648P the mutant has full catalytic activity for nisin leader-peptide cleavage, although the C-terminal region of the enzyme is no longer cleaved Lactococcus lactis
3.4.22.46 S512A inactive Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.46 72900
-
 x * 72900, enzyme with the C-terminus cleaved off, SDS-PAGE Lactococcus lactis
3.4.22.46 77500
-
 x * 77500, enzyme with the C-terminus intact, SDS-PAGE Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.46 additional information Lactococcus lactis the enzyme possesses an auto-cleavage site between residues Arg647 and Ser648 ?
-
 ?
3.4.22.46 nisin + H2O Lactococcus lactis the final step in nisin maturation is the proteolytic cleavage of the first 23 amino-terminal residues by NisP ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.46 Lactococcus lactis D9IXC0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.46 Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.46 Edans-EGAVSVRSQEIK-Dabcyl + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.22.46 additional information the enzyme possesses an auto-cleavage site between residues Arg647 and Ser648 Lactococcus lactis ?
-
 ?
3.4.22.46 nisin + H2O the final step in nisin maturation is the proteolytic cleavage of the first 23 amino-terminal residues by NisP Lactococcus lactis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.46 ? x * 72900, enzyme with the C-terminus cleaved off, SDS-PAGE Lactococcus lactis
3.4.22.46 ? x * 77500, enzyme with the C-terminus intact, SDS-PAGE Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
3.4.22.46 nisin leader peptidase
-
 Lactococcus lactis
3.4.22.46 NisP
-
 Lactococcus lactis

General Information

EC Number General Information Comment Organism
3.4.22.46 metabolism functions in the last step of nisin maturation as the leader-peptide peptidase Lactococcus lactis