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Literature summary extracted from

  • Kord, M.; Youssef, E.; Ahmed, H.; Qaid, E.
    Purification and characterization of trehalase from seeds of chickpea (Cicer arietinum L.) (2013), Turk. J. Biol., 37, 661-669.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 ATP 10% residual activity at 20 mM Cicer arietinum
3.2.1.28 Borate 16% residual activity at 20 mM Cicer arietinum
3.2.1.28 Ca2+ 67% residual activity at 20 mM Cicer arietinum
3.2.1.28 Cu2+ 14% residual activity at 20 mM Cicer arietinum
3.2.1.28 EDTA 16% residual activity at 20 mM Cicer arietinum
3.2.1.28 Fe3+ 31% residual activity at 20 mM Cicer arietinum
3.2.1.28 K+ 69% residual activity at 20 mM Cicer arietinum
3.2.1.28 Li+ 12% residual activity at 20 mM Cicer arietinum
3.2.1.28 malate 37% residual activity at 20 mM Cicer arietinum
3.2.1.28 mannitol competitive inhibitor, 8% residual activity at 20 mM Cicer arietinum
3.2.1.28 Na+ 61% residual activity at 20 mM Cicer arietinum
3.2.1.28 succinate 41% residual activity at 20 mM Cicer arietinum
3.2.1.28 validamycin A competitive inhibitor, 49% residual activity at 5 nM Cicer arietinum
3.2.1.28 Zn2+ non-competitive inhibitor, 22% residual activity at 20 mM Cicer arietinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 3.64
-
 alpha,alpha-trehalose at pH 5.5 and 55°C Cicer arietinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Co2+ 122% activity at 20 mM Cicer arietinum
3.2.1.28 Mg2+ 133% activity at 20 mM Cicer arietinum
3.2.1.28 Mn2+ 210% activity at 20 mM Cicer arietinum
3.2.1.28 Ni2+ 122% activity at 20 mM Cicer arietinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 38000
-
 6 * 38000, SDS-PAGE Cicer arietinum
3.2.1.28 223000
-
 gel filtration Cicer arietinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O Cicer arietinum
-
 beta-D-glucose + alpha-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Cicer arietinum
-
 cultivar Giza 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 ammonium sulfate precipitation, DEAE-cellulose column chromatography, CM Sepharose column chromatography, and Sephadex G-200 gel filtration Cicer arietinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 seed
-
 Cicer arietinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 0.5
-
 crude extract, at pH 5.5 and 55°C Cicer arietinum
3.2.1.28 117
-
 after 232fold purification, at pH 5.5 and 55°C Cicer arietinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Cicer arietinum beta-D-glucose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 homohexamer 6 * 38000, SDS-PAGE Cicer arietinum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 trehalase
-
 Cicer arietinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 60
-
-
 Cicer arietinum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.28 35 65 more than 50% activity between 35 and 65°C Cicer arietinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 5 5.5
-
 Cicer arietinum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.28 0.000004
-
 validamycin A at pH 5.5 and 55°C Cicer arietinum
3.2.1.28 7
-
 Zn2+ at pH 5.5 and 55°C Cicer arietinum
3.2.1.28 9
-
 mannitol at pH 5.5 and 55°C Cicer arietinum