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Literature summary extracted from

  • Borchani, L.; Sassi, A.; Ben Gharsa, H.; Safra, I.; Shahbazzadeh, D.; Ben Lasfar, Z.; El Ayeb, M.
    The pathological effects of heminecrolysin, a dermonecrotic toxin from Hemiscorpius lepturus scorpion venom are mediated through its lysophospholipase D activity (2013), Toxicon, 68, 30-39.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.39 1,10-phenantroline 50% inhibition at 2.5 mM, 70% inhibition Hemiscorpius lepturus
3.1.4.39 EDTA 98% inhibition Hemiscorpius lepturus
3.1.4.39 EGTA 48% inhibition Hemiscorpius lepturus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.39 0.097
-
 1-alkyl-sn-glycero-3-phosphocholine pH 7.4, 37°C Hemiscorpius lepturus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.39 extracellular
-
 Hemiscorpius lepturus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.39 Ca2+ activates, dependent on Hemiscorpius lepturus
3.1.4.39 Mg2+ activates, dependent on Hemiscorpius lepturus
3.1.4.39 Zn2+ activates Hemiscorpius lepturus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.39 33000
-
 x * 33000 Hemiscorpius lepturus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.39 1-alkyl-sn-glycero-3-phosphocholine + H2O Hemiscorpius lepturus
-
 1-alkyl-sn-glycerol 3-phosphate + choline
-
 ?
3.1.4.39 additional information Hemiscorpius lepturus the enzyme is active on human macrophages, erythrocytes, Jurkat and HeLa cells, and on chicken embryo chorioallantoic membrane ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.39 Hemiscorpius lepturus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.39 venom
-
 Hemiscorpius lepturus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.39 1-alkyl-sn-glycero-3-phosphocholine + H2O
-
 Hemiscorpius lepturus 1-alkyl-sn-glycerol 3-phosphate + choline
-
 ?
3.1.4.39 additional information the enzyme is active on human macrophages, erythrocytes, Jurkat and HeLa cells, and on chicken embryo chorioallantoic membrane Hemiscorpius lepturus ?
-
 ?
3.1.4.39 additional information the enzyme shows slight activity with sphingomyelin, but no activity with phosphatidylcholine Hemiscorpius lepturus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.39 ? x * 33000 Hemiscorpius lepturus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.39 Heminecrolysin
-
 Hemiscorpius lepturus
3.1.4.39 lysophospholipase D
-
 Hemiscorpius lepturus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.39 37
-
 assay at Hemiscorpius lepturus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.39 7.4
-
 assay at Hemiscorpius lepturus

General Information

EC Number General Information Comment Organism
3.1.4.39 additional information Heminecrolysin does not affect glycophorin A expression Hemiscorpius lepturus
3.1.4.39 physiological function Heminecrolysin induces erythrocyte hemolysis via lysophospholipase D activity. Heminecrolysin efficiently binds to erythrocyte's membrane and provokes phosphatidylserine translocation without cleavage of glycophorin A, suggesting that, unlike spider's phospholipase D, complement is activated only via the classical pathway. Heminecrolysin induces pro-inflammatory cytokine release and causes necrosis in the vascular tissue Hemiscorpius lepturus