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Literature summary extracted from

  • Wright, J.R.; Keffer-Wilkes, L.C.; Dobing, S.R.; Kothe, U.
    Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis (2011), RNA, 17, 2074-2084.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.70 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.70 additional information Escherichia coli the enzyme catalyzes pseudouridylation at position 55 in the T-pseudouridine-C arm of all elongator tRNAs ?
-
 ?
4.2.1.70 uracil + D-ribose 5-phosphate Escherichia coli
-
 pseudouridine 5'-phosphate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.70 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.70 Ni2+ Sepharose column chromatography and Superdex 75 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.70 additional information the enzyme catalyzes pseudouridylation at position 55 in the T-pseudouridine-C arm of all elongator tRNAs Escherichia coli ?
-
 ?
4.2.1.70 uracil + D-ribose 5-phosphate
-
 Escherichia coli pseudouridine 5'-phosphate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.70 pseudouridine synthase
-
 Escherichia coli
4.2.1.70 TruB
-
 Escherichia coli