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Literature summary extracted from

  • Atkinson, S.; Hor, L.; Dogovski, C.; Dobson, R.; Perugini, M.
    Identification of the bona fide DHDPS from a common plant pathogen (2014), Proteins, 82, 1869-1883.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.3.7 drug development the enzyme is a bona fide drug target for treatment of the Crown Gall disease on crops caused by Agrobacterium tumefaciens, rational design of pesticide agents Agrobacterium tumefaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.7 gene dapA1, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA10, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA2, sequence comparisons, expression of the His-tagged isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA3, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA4, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA5, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA6, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA7, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA8, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
4.3.3.7 gene dapA9, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.7 crystal structure analysis, PDB ID 2HMC, structure comparisons Agrobacterium tumefaciens
4.3.3.7 crystal structure analysis, PDB ID 2R8Wm, structure comparisons Agrobacterium tumefaciens
4.3.3.7 crystal structure analysis, PDB ID 3B4U, structure comparisons Agrobacterium tumefaciens
4.3.3.7 crystal structure analysis, PDB IDs 4I7U, 4I7V, and 4I7W, resolution at 1.42-1.69 A, structure comparisons Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.7 (S)-aspartate-4-semialdehyde substrate inhibition Agrobacterium tumefaciens
4.3.3.7 (S)-lysine allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling Agrobacterium tumefaciens
4.3.3.7 pyruvate substrate inhibition Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.7 0.16 0.32 (S)-aspartate-4-semialdehyde pH and temperature not specified in the publication Agrobacterium tumefaciens
4.3.3.7 0.31 0.44 pyruvate pH and temperature not specified in the publication Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate Agrobacterium tumefaciens
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate Agrobacterium tumefaciens C58 / ATCC 33970
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Agrobacterium tumefaciens A9CFV4 dapA10; gene dapA10
-
4.3.3.7 Agrobacterium tumefaciens A9CGZ4 dapA8; gene dapA8
-
4.3.3.7 Agrobacterium tumefaciens A9CHR2 dapA5; gene dapA5
-
4.3.3.7 Agrobacterium tumefaciens A9CL94 dapA2; genes dapA2
-
4.3.3.7 Agrobacterium tumefaciens A9CL97 dapA3; gene dapA3
-
4.3.3.7 Agrobacterium tumefaciens Q7CU96 dapA9; gene dapA9
-
4.3.3.7 Agrobacterium tumefaciens Q7D0E8 dapA6; gene dapA6
-
4.3.3.7 Agrobacterium tumefaciens Q7D313 dapA1; gene dapA1
-
4.3.3.7 Agrobacterium tumefaciens Q7D3Z9 dapA4; gene dapA4
-
4.3.3.7 Agrobacterium tumefaciens Q8UGL3 dapA7; gene dapA7
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 A9CFV4 dapA10; gene dapA10
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 A9CGZ4 dapA8; gene dapA8
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 A9CHR2 dapA5; gene dapA5
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 A9CL94 dapA2; genes dapA2
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 A9CL97 dapA3; gene dapA3
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 Q7CU96 dapA9; gene dapA9
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 Q7D0E8 dapA6; gene dapA6
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 Q7D313 dapA1; gene dapA1
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 Q7D3Z9 dapA4; gene dapA4
-
4.3.3.7 Agrobacterium tumefaciens C58 / ATCC 33970 Q8UGL3 dapA7; gene dapA7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7 recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate
-
 Agrobacterium tumefaciens (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate bi-bi ping-pong substrate model Agrobacterium tumefaciens (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate
-
 Agrobacterium tumefaciens C58 / ATCC 33970 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate bi-bi ping-pong substrate model Agrobacterium tumefaciens C58 / ATCC 33970 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.7 homotetramer the enzyme adopts the canonical homotetrameric structure in both solution and the crystal state Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
4.3.3.7 DHDPS
-
 Agrobacterium tumefaciens
4.3.3.7 dihydrodipicolinate synthase
-
 Agrobacterium tumefaciens
4.3.3.7 HTPA synthase UniProt Agrobacterium tumefaciens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.3.7 0.15
-
 (S)-aspartate-4-semialdehyde pH and temperature not specified in the publication Agrobacterium tumefaciens
4.3.3.7 0.19 0.57 pyruvate pH and temperature not specified in the publication Agrobacterium tumefaciens

General Information

EC Number General Information Comment Organism
4.3.3.7 metabolism the enzyme catalyses the first committed step in the lysine biosynthesis pathway, the condensation of pyruvate and (S)-aspartate semialdehyde to form (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid Agrobacterium tumefaciens
4.3.3.7 additional information the catalytic triad, consisting of Tyr133, Thr44, and Tyr107, acts as a proton relay to transfer protons to and from the active site via a water-filled channel leading to bulk solvent Agrobacterium tumefaciens