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Literature summary extracted from

  • McTernan, P.M.; Chandrayan, S.K.; Wu, C.H.; Vaccaro, B.J.; Lancaster, W.A.; Adams, M.W.
    Engineering the respiratory membrane-bound hydrogenase of the hyperthermophilic archaeon Pyrococcus furiosus and characterization of the catalytically active cytoplasmic subcomplex (2015), Protein Eng. Des. Sel., 28, 1-8.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.12.7.2 additional information engineering and expressing of a His-tagged minimal catalytically active 4-subunit cytoplasmic subcomplex of respiratory membrane-bound hydrogenase. In order to obtain a minimal subcomplex, the native MBH operon is splitt it into two separate transcriptional units Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.7.2 cytoplasm minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase Pyrococcus furiosus 5737
-
1.12.7.2 membrane native 14-subunit membrane-bound subcomplex of respiratory membrane-bound hydrogenase Pyrococcus furiosus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe contains [NiFe]-clusters. The Fe: Ni ratio of the engineered and expressed His-tagged 4-subunit subcomplex of respiratory membrane-bound hydrogenase. Subunit MbhL harbors the [NiFe] active site, subunit MbhK is part of the large subunit, subunit MbhJ harbors one [4Fe4S] cluster and MbhN harbors two [4Fe4S] clusters Pyrococcus furiosus
1.12.7.2 Ni contains [NiFe]-clusters. The Fe: Ni ratio of the engineered and expressed His-tagged 4-subunit subcomplex of respiratory membrane-bound hydrogenase. Subunit MbhL harbors the [NiFe] active site, subunit MbhK is part of the large subunit, subunit MbhJ harbors one [4Fe4S] cluster and MbhN harbors two [4Fe4S] clusters Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 85000
-
 gel filtration, engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Pyrococcus furiosus Q8U0Z8 and Q8U0Z7 and Q8U0Z6 Q8U0Z8: subunit mbhJ, Q8U0Z7: subunit mbhK, Q8U0Z6: catalytic subunit mbhL, subunit mbhN. Engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, MbhJKLN
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.7.2 the O2 sensitivities of minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase and the native 14-subunit membrane-bound version are similar, half-lives of about 15 h in air Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 purification of the engineered and expressed His-tagged 4-subunit cytoplasmic subcomplex of respiratory membrane-bound hydrogenase Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + 2 oxidized ferredoxin
-
 Pyrococcus furiosus 2 reduced ferredoxin + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 C-MBH engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase Pyrococcus furiosus
1.12.7.2 MbhJKLN engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, MbhJKLN Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 60
-
 the minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, H2 formation with the physiological electron donor, reduced ferredoxin Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.7.2 90
-
 half-life of minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase: 8 h. Half-life of the native 14-subunit membrane-bound respiratory membrane-bound hydrogenase complex Pyrococcus furiosus