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Literature summary extracted from

  • San Francisco, B.; Bretsnyder, E.C.; Kranz, R.G.
    Human mitochondrial holocytochrome c synthases heme binding, maturation determinants, and complex formation with cytochrome c (2013), Proc. Natl. Acad. Sci. USA, 110, E788-E797.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.17 coexpression of N-terminally GST-tagged wild-type enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants in Escherichia coli Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.17 mitochondrial membrane membrane-bound Homo sapiens 31966
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.17 57000
-
 x * 57000, recombinant GST-tagged enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.17 Apocytochrome c + heme Homo sapiens
-
 Holocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.17 Homo sapiens P53701
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.17 wild-type membrane-bound human GST-tagged enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants from Escherichia coli Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.17 Apocytochrome c + heme
-
 Homo sapiens Holocytochrome c
-
 ?
4.4.1.17 Apocytochrome c + heme conserved His154 is the key ligand to the heme iron, formation of the enzyme-heme complex serves as the platform for interaction with apocytochrome c, the heme is the central molecule mediating contact between enzyme and apocytochrome c. Conserved His19 of the CXXCH motif in apocytochrome c supplies the second axial ligand to heme in the trapped enzyme-heme-cytochrome c complex. Molecular mechanisms, overview Homo sapiens Holocytochrome c
-
 ?
4.4.1.17 additional information substrate specificity of the human enzyme, overview. Engineering of a bacterial cytochrome c into a robust substrate for the human enzyme, overview Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.17 ? x * 57000, recombinant GST-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.4.1.17 HCCS
-
 Homo sapiens
4.4.1.17 Holocytochrome c synthetase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
4.4.1.17 physiological function cytochrome c, an essential electron carrier in mitochondria and a critical component of the apoptotic pathway, contains a heme cofactor covalently attached to the protein at a conserved CXXCH motif requiring the mitochondrial protein holocytochrome c synthase Homo sapiens