EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.12 | expressed in Escherichia coli T7 Express cells | Brugia malayi |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.3.12 | hanging drop vapor diffusion method, using 33% (w/v) PEG 300, 0.1 M sodium citrate pH 5.0, 13% ethylene glycol and 10 mM CoCl2 | Brugia malayi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.12 | D213A | inactive | Brugia malayi |
3.1.3.12 | D215A | inactive | Brugia malayi |
3.1.3.12 | D336A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | D378A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | DELTA59 | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | E384A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | E386A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | K334A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | N228A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | Q332A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | R337A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | S329A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | T339A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | W280A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | Y221A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
3.1.3.12 | Y225A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Brugia malayi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.12 | D-glucose 6-sulfate | - |
Brugia malayi | |
3.1.3.12 | trehalose | - |
Brugia malayi | |
3.1.3.12 | trehalose 6-sulfate | competitive inhibitor | Brugia malayi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.12 | 0.04 | - |
trehalose 6-phosphate | mutant enzyme N228A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.046 | - |
trehalose 6-phosphate | mutant enzyme D336A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.082 | - |
trehalose 6-phosphate | mutant enzyme E386A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.1 | - |
trehalose 6-phosphate | mutant enzyme E384A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.15 | - |
trehalose 6-phosphate | mutant enzyme W280A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.15 | - |
trehalose 6-phosphate | mutant enzyme Y225A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.2 | - |
trehalose 6-phosphate | mutant enzyme Q332A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.2 | - |
trehalose 6-phosphate | mutant enzyme T339A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.25 | - |
trehalose 6-phosphate | mutant enzyme S329A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.26 | - |
trehalose 6-phosphate | mutant enzyme DELTA59, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.27 | - |
trehalose 6-phosphate | mutant enzyme D378A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.33 | - |
trehalose 6-phosphate | mutant enzyme Y221A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.36 | - |
trehalose 6-phosphate | wild type enzyme, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.4 | - |
trehalose 6-phosphate | mutant enzyme R337A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.5 | - |
trehalose 6-phosphate | mutant enzyme K334A, at pH 7.5 and 22°C | Brugia malayi |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.12 | Mg2+ | required | Brugia malayi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.12 | trehalose 6-phosphate + H2O | Brugia malayi | - |
trehalose + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.12 | Brugia malayi | A8NS89 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.12 | HiTrap Q column chromatography | Brugia malayi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.12 | trehalose 6-phosphate + H2O | - |
Brugia malayi | trehalose + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.12 | GOB-1 | - |
Brugia malayi |
3.1.3.12 | T6PP | - |
Brugia malayi |
3.1.3.12 | trehalose 6-phosphate phosphatase | - |
Brugia malayi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.12 | 0.06 | - |
trehalose 6-phosphate | mutant enzyme K334A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.094 | - |
trehalose 6-phosphate | mutant enzyme D336A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.22 | - |
trehalose 6-phosphate | mutant enzyme Q332A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.28 | - |
trehalose 6-phosphate | mutant enzyme R337A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 1 | - |
trehalose 6-phosphate | mutant enzyme Y221A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 2 | 8 | trehalose 6-phosphate | mutant enzyme DELTA59, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 2 | - |
trehalose 6-phosphate | mutant enzyme E386A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 3 | - |
trehalose 6-phosphate | mutant enzyme E384A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 4 | - |
trehalose 6-phosphate | mutant enzyme S329A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 6 | - |
trehalose 6-phosphate | mutant enzyme Y225A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 11 | - |
trehalose 6-phosphate | mutant enzyme D378A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 15 | - |
trehalose 6-phosphate | mutant enzyme T339A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 18 | - |
trehalose 6-phosphate | mutant enzyme N228A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 19 | - |
trehalose 6-phosphate | mutant enzyme W280A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 24 | - |
trehalose 6-phosphate | wild type enzyme, at pH 7.5 and 22°C | Brugia malayi |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.12 | 0.082 | - |
trehalose 6-sulfate | wild type enzyme, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 10 | - |
trehalose | Ki above 10 mM, wild type enzyme, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 10 | - |
D-glucose 6-sulfate | Ki above 10 mM, wild type enzyme, at pH 7.5 and 22°C | Brugia malayi |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.12 | 0.11 | - |
trehalose 6-phosphate | mutant enzyme K334A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.2 | - |
trehalose 6-phosphate | mutant enzyme D336A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 0.55 | - |
trehalose 6-phosphate | mutant enzyme Q332A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 1.5 | - |
trehalose 6-phosphate | mutant enzyme R337A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 3 | - |
trehalose 6-phosphate | mutant enzyme Y221A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 15 | - |
trehalose 6-phosphate | mutant enzyme S329A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 28 | - |
trehalose 6-phosphate | mutant enzyme E386A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 30 | - |
trehalose 6-phosphate | mutant enzyme E384A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 36 | - |
trehalose 6-phosphate | mutant enzyme Y225A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 39 | - |
trehalose 6-phosphate | mutant enzyme D378A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 69 | - |
trehalose 6-phosphate | wild type enzyme, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 77 | - |
trehalose 6-phosphate | mutant enzyme T339A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 110 | - |
trehalose 6-phosphate | mutant enzyme DELTA59, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 120 | - |
trehalose 6-phosphate | mutant enzyme W280A, at pH 7.5 and 22°C | Brugia malayi | |
3.1.3.12 | 450 | - |
trehalose 6-phosphate | mutant enzyme N228A, at pH 7.5 and 22°C | Brugia malayi |