EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | 4-nitrophenyl phosphate | competitive | Nicotiana tabacum | |
3.1.3.2 | ATP | 1 mM, 29% residual activity, competitive | Nicotiana tabacum | |
3.1.3.2 | EDTA | 1 mM, 62% residual activity | Nicotiana tabacum | |
3.1.3.2 | fluoride | 1 mM, 56% residual activity | Nicotiana tabacum | |
3.1.3.2 | molybdate | 1 mM, 5% residual activity | Nicotiana tabacum | |
3.1.3.2 | additional information | not inhibitory: levamisole and tartrate | Nicotiana tabacum | |
3.1.3.2 | phosphate | 1 mM, 20% residual activity, competitive | Nicotiana tabacum | |
3.1.3.2 | pyridoxal | 1 mM, 20% residual activity, competitive | Nicotiana tabacum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.2 | 0.24 | - |
pyridoxal 5'-phosphate | pH 5.5, 37°C | Nicotiana tabacum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | Ca2+ | 1 mM, 179% of initial activity | Nicotiana tabacum | |
3.1.3.2 | Mg2+ | most effective dimetal ion for catalysis under saturating substrate concentrations, 1 mM, 215% of initial activity | Nicotiana tabacum | |
3.1.3.2 | Mn2+ | 1 mM, 154% of initial activity | Nicotiana tabacum | |
3.1.3.2 | additional information | a divalent metal ion is required for activity | Nicotiana tabacum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 24900 | - |
2 * 24900, SDS-PAGE | Nicotiana tabacum |
3.1.3.2 | 50000 | - |
gel filtration | Nicotiana tabacum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.2 | Nicotiana tabacum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.2 | - |
Nicotiana tabacum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.2 | leaf | - |
Nicotiana tabacum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 1998 | - |
pH 5.5, 37°C | Nicotiana tabacum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.2 | 4-nitrophenyl phosphate + H2O | - |
Nicotiana tabacum | 4-nitrophenol + phosphate | about 158% of the activity with pyridoxal 5'-phosphate | ? | |
3.1.3.2 | diphosphate + H2O | - |
Nicotiana tabacum | 2 phosphate | about 156% of the activity with pyridoxal 5'-phosphate | ? | |
3.1.3.2 | pyridoxal 5'-phosphate + H2O | - |
Nicotiana tabacum | pyridoxal + phosphate | - |
? | |
3.1.3.2 | pyridoxamine 5'-phosphate + H2O | - |
Nicotiana tabacum | pyridoxamine + phosphate | about 50% of the activity with pyridoxal 5'-phosphate | ? | |
3.1.3.2 | pyridoxine 5'-phosphate + H2O | - |
Nicotiana tabacum | pyridoxine + phosphate | about 50% of the activity with pyridoxal 5'-phosphate | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.2 | dimer | 2 * 24900, SDS-PAGE | Nicotiana tabacum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 50 | - |
- |
Nicotiana tabacum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 37 | - |
citric acid-sodium citrate buffer, pH 5.5, fully stable at least for 120 min | Nicotiana tabacum |
3.1.3.2 | 50 | - |
half-life around 50 min | Nicotiana tabacum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 5.5 | - |
- |
Nicotiana tabacum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 5 | 6 | 37°C for 2 h, more than 80% of total activities | Nicotiana tabacum |