BRENDA - Enzyme Database show

Medicago truncatula dihydrodipicolinate synthase (DHDPS) enzymes display novel regulatory properties

Erzeel, E.; Van Bochaute, P.; Thu, T.T.; Angenon, G.; Plant Mol. Biol. 81, 401-415 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.3.7
isozymes MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4, phylogenetic analysis, quantitative RT-PCR expression analysis, the different isogenes are transcriptionally regulated in an organ-specific manner, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), coexpresion of His-tagged isozyme MtDHDPS3 with N-terminally FLAG-tagged enzyme AtDHDPS2 from Arabidopsis thliana in Escherichia coli strain BL21(DE3), expression of isozyme MtDHDPS3 in transgenic Arabidopsis thaliana plants under the control of a constitutive 35S promoter via Agrobacterium tumefaciens transfection resulting in an inhibition of enzyme activity
Medicago truncatula
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-lysine
feedback inhibition
Medicago truncatula
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Medicago truncatula
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Medicago truncatula Jemalong 2HA
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Medicago truncatula
-
four isozymes, MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4
-
4.3.3.7
Medicago truncatula Jemalong 2HA
-
four isozymes, MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
recombinant His-tagged isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Medicago truncatula
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
leaf
-
Medicago truncatula
-
4.3.3.7
additional information
organ-specific isozyme expression
Medicago truncatula
-
4.3.3.7
root
isozyme MtDHDPS2 shows highest expression in roots
Medicago truncatula
-
4.3.3.7
seed
isozymes MtDHDPS1, MtDHDPS3, and MtDHDPS4 shows highest expression in mature seeds
Medicago truncatula
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
additional information
isozyme MtDHDPS1, followed by isozyme MtDHDPS4, exhibits the highest activity, while activity of isozyme MtDHDPS2 and especially of isozyme MtDHDPS3 is low
730623
Medicago truncatula
?
-
-
-
-
4.3.3.7
additional information
isozyme MtDHDPS1, followed by isozyme MtDHDPS4, exhibits the highest activity, while activity of isozyme MtDHDPS2 and especially of isozyme MtDHDPS3 is low
730623
Medicago truncatula Jemalong 2HA
?
-
-
-
-
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
730623
Medicago truncatula
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
730623
Medicago truncatula Jemalong 2HA
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.3.7
37
-
assay at
Medicago truncatula
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
isozymes MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4, phylogenetic analysis, quantitative RT-PCR expression analysis, the different isogenes are transcriptionally regulated in an organ-specific manner, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), coexpresion of His-tagged isozyme MtDHDPS3 with N-terminally FLAG-tagged enzyme AtDHDPS2 from Arabidopsis thliana in Escherichia coli strain BL21(DE3), expression of isozyme MtDHDPS3 in transgenic Arabidopsis thaliana plants under the control of a constitutive 35S promoter via Agrobacterium tumefaciens transfection resulting in an inhibition of enzyme activity
Medicago truncatula
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-lysine
feedback inhibition
Medicago truncatula
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Medicago truncatula
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Medicago truncatula Jemalong 2HA
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
recombinant His-tagged isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Medicago truncatula
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
leaf
-
Medicago truncatula
-
4.3.3.7
additional information
organ-specific isozyme expression
Medicago truncatula
-
4.3.3.7
root
isozyme MtDHDPS2 shows highest expression in roots
Medicago truncatula
-
4.3.3.7
seed
isozymes MtDHDPS1, MtDHDPS3, and MtDHDPS4 shows highest expression in mature seeds
Medicago truncatula
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
additional information
isozyme MtDHDPS1, followed by isozyme MtDHDPS4, exhibits the highest activity, while activity of isozyme MtDHDPS2 and especially of isozyme MtDHDPS3 is low
730623
Medicago truncatula
?
-
-
-
-
4.3.3.7
additional information
isozyme MtDHDPS1, followed by isozyme MtDHDPS4, exhibits the highest activity, while activity of isozyme MtDHDPS2 and especially of isozyme MtDHDPS3 is low
730623
Medicago truncatula Jemalong 2HA
?
-
-
-
-
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
730623
Medicago truncatula
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
730623
Medicago truncatula Jemalong 2HA
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.3.7
37
-
assay at
Medicago truncatula
General Information
EC Number
General Information
Commentary
Organism
4.3.3.7
metabolism
lysine biosynthesis in plants is tightly regulated by feedback inhibition of the end product on dihydrodipicolinate synthase, the first enzyme of the lysine-specific branch
Medicago truncatula
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.3.3.7
metabolism
lysine biosynthesis in plants is tightly regulated by feedback inhibition of the end product on dihydrodipicolinate synthase, the first enzyme of the lysine-specific branch
Medicago truncatula