Literature summary extracted from

Guennewich, N.; Higashi, Y.; Feng, X.; Choi, K.B.; Schmidt, J.; Kutchan, T.M.
A diterpene synthase from the clary sage Salvia sclarea catalyzes the cyclization of geranylgeranyl diphosphate to (8R)-hydroxy-copalyl diphosphate (2013), Phytochemistry, 91, 93-99.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.3.133	89758	-	x * 89758, calulated	Salvia sclarea

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.3.133	Salvia sclarea	J9PDV7	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.3.133	geranylgeranyl diphosphate + H2O	it is proposed that a basic amino acid residue of the enzyme abstracts a proton from a molecule of water as the hydroxyl adds to the double bond at carbon7 of geranylgeranyl diphosphate. Concerted cyclization occurs and the double bond at carbon14 of geranylgeranyl diphosphate (carbon-3 of (8R)-hydroxy-copalyl diphosphate) is protonated. This concerted mechanism accounts for the addition of a single atom of 18O and a single atom of deuterium to geranylgeranyl diphosphate to form (8R)-hydroxy-copalyl diphosphate	Salvia sclarea	(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate	first step in synthesis of sclareol	?

Subunits

EC Number	Subunits	Comment	Organism
4.2.3.133	?	x * 89758, calulated	Salvia sclarea

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.3.133	TPS1	-	Salvia sclarea

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Release 2023.1 (January 2023)