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Literature summary extracted from

  • Kurzban, G.P.; Chu, L.; Ebersole, J.L.; Holt, S.C.
    Sulfhemoglobin formation in human erythrocytes by cystalysin, an L-cysteine desulfhydrase from Treponema denticola (1999), Oral Microbiol. Immunol., 14, 153-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.28 expression in Escherichia coli Treponema denticola

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.28 Cyanoborohydride complete inactivation Treponema denticola
4.4.1.28 glycine competitive inhibitor Treponema denticola
4.4.1.28 L-alanine competitive inhibitor Treponema denticola
4.4.1.28 L-tryptophan competitive inhibitor Treponema denticola
4.4.1.28 pyruvate
-
 Treponema denticola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.28 0.28
-
 S-ethyl-L-cysteine pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola
4.4.1.28 0.36
-
 beta-chloro-L-alanine pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola
4.4.1.28 0.4
-
 L-cysteine pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola
4.4.1.28 1.26
-
 S-methyl-L-cysteine pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola
4.4.1.28 2.3
-
 L-cysteine pH and temperature not specified in the publication, in absence of Brij 35 Treponema denticola
4.4.1.28 62
-
 L-cysteine methyl ester pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.28 L-cysteine + H2O Treponema denticola the enzyme is responsible for in vivo cysteine catabolism by Treponema denticola sulfide + NH3 + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.28 Treponema denticola
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.28
-
 Treponema denticola

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.28 beta-chloro-L-alanine + H2O
-
 Treponema denticola sulfide + ?
-
 ?
4.4.1.28 L-cysteine + H2O the enzyme is responsible for in vivo cysteine catabolism by Treponema denticola Treponema denticola sulfide + NH3 + pyruvate
-
 ?
4.4.1.28 L-cysteine + H2O strong preference for L-cysteine over D-cysteine, H2S production is 483-fold slower with D-cysteine than with L-cysteine. It is far more active towards L-cysteine than towards the other standard amino acids that undergo pyridoxal phosphate-dependent beta-elimination reactions (serine, threonine, tryptophan and tyrosine). The enzyme tolerated small modifications to the carboxylate of L-cysteine (i.e., the methyl and ethyl esters of L-cysteine are good substrates), but the smallest possible peptide with an N-terminal cysteine, L-cysteinylglycine, is a very poor substrate Treponema denticola sulfide + NH3 + pyruvate
-
 ?
4.4.1.28 L-cysteine methyl ester + H2O
-
 Treponema denticola sulfide + ?
-
 ?
4.4.1.28 S-ethyl-L-cysteine + H2O
-
 Treponema denticola sulfide + ?
-
 ?
4.4.1.28 S-methyl-L-cysteine + H2O
-
 Treponema denticola sulfide + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.28 cystalysin
-
 Treponema denticola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.28 5.5
-
 L-cysteine pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola
4.4.1.28 5.95
-
 S-methyl-L-cysteine pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola
4.4.1.28 9.5
-
 S-ethyl-L-cysteine pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola
4.4.1.28 10.9
-
 L-cysteine pH and temperature not specified in the publication, in absence of Brij 35 Treponema denticola
4.4.1.28 14.5
-
 beta-chloro-L-alanine pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola
4.4.1.28 32
-
 L-cysteine methyl ester pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer Treponema denticola

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.28 pyridoxal 5'-phosphate dependent on Treponema denticola

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.28 12
-
 L-tryptophan pH and temperature not specified in the publication, in absence of Brij 35 Treponema denticola

General Information

EC Number General Information Comment Organism
4.4.1.28 metabolism the enzyme is responsible for in vivo cysteine catabolism by Treponema denticola. Unregulated enzyme, with the resulting H2S production being essential to the hemolytic activity Treponema denticola