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Literature summary extracted from
- A novel endonuclease that may be responsible for damaged DNA base repair in Pyrococcus furiosus (2015), Nucleic Acids Res., 43, 2853-2863.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.B1 | expression in Escherichia coli | Pyrococcus furiosus |
| 3.1.21.B1 | expression in Escherichia coli | Thermococcus kodakarensis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.B1 | 47653 | - |
x * 47653, calculated from sequence | Pyrococcus furiosus |
| 3.1.21.B1 | 48080 | - |
x * 48080, calculated from sequence | Thermococcus kodakarensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.B1 | deoxyinosine-containing DNA + H2O | Pyrococcus furiosus | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The enzyme is an endonuclease that functions in a repair process for damaged bases in archaeal cells | ? | - |
? |  |
| 3.1.21.B1 | deoxyinosine-containing DNA + H2O | Thermococcus kodakarensis | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The enzyme is an endonuclease that functions in a repair process for damaged bases in archaeal cells | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.B1 | Pyrococcus furiosus | Q8U0N5 | - |
- |
| 3.1.21.B1 | Thermococcus kodakarensis | Q5JI33 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.B1 | - |
Pyrococcus furiosus |
| 3.1.21.B1 | - |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.B1 | 7-deaza-2'-deoxyxanthosine-containing DNA + H2O | normal ssDNA, dsDNA and the DNA containing a G/T mismatch are completely resistant to the cleavage reaction. The lengths of the products indicate that PfuEndoQ cleaves DNA on the 5'-side of the lesions | Pyrococcus furiosus | ? | - |
? | |
| 3.1.21.B1 | 7-deaza-2'-deoxyxanthosine-containing DNA + H2O | the enzyme cleaves a ligatable nick (5'-phosphate and 3'-hydroxyl termini) on substrates after the cleavage | Thermococcus kodakarensis | ? | - |
? | |
| 3.1.21.B1 | apurinic/apyrimidinic site-containing DNA + H2O | normal ssDNA, dsDNA and the DNA containing a G/T mismatch are completely resistant to the cleavage reaction. The lengths of the products indicate that PfuEndoQ cleaves DNA on the 5'-side of the lesions | Pyrococcus furiosus | ? | - |
? | |
| 3.1.21.B1 | apurinic/apyrimidinic site-containing DNA + H2O | the enzyme cleaves a ligatable nick (5'-phosphate and 3'-hydroxyl termini) on substrates after the cleavage | Thermococcus kodakarensis | ? | - |
? | |
| 3.1.21.B1 | deoxyinosine-containing DNA + H2O | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The enzyme is an endonuclease that functions in a repair process for damaged bases in archaeal cells | Pyrococcus furiosus | ? | - |
? | |
| 3.1.21.B1 | deoxyinosine-containing DNA + H2O | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The enzyme is an endonuclease that functions in a repair process for damaged bases in archaeal cells | Thermococcus kodakarensis | ? | - |
? | |
| 3.1.21.B1 | deoxyinosine-containing DNA + H2O | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine | Thermococcus kodakarensis | ? | - |
? | |
| 3.1.21.B1 | deoxyinosine-containing DNA + H2O | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The normal ssDNA, dsDNA and the DNA containing a G/T mismatch are completely resistant to the cleavage reaction. The lengths of the products indicate that PfuEndoQ cleaves DNA on the 5'-side of the lesions | Pyrococcus furiosus | ? | - |
? | |
| 3.1.21.B1 | deoxyuridine-containing DNA + H2O | normal ssDNA, dsDNA and the DNA containing a G/T mismatch are completely resistant to the cleavage reaction. The lengths of the products indicate that PfuEndoQ cleaves DNA on the 5'-side of the lesions | Pyrococcus furiosus | ? | - |
? | |
| 3.1.21.B1 | deoxyuridine-containing DNA + H2O | the enzyme cleaves a ligatable nick (5'-phosphate and 3'-hydroxyl termini) on substrates after the cleavage | Thermococcus kodakarensis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.21.B1 | ? | x * 48080, calculated from sequence | Thermococcus kodakarensis |
| 3.1.21.B1 | ? | x * 47653, calculated from sequence | Pyrococcus furiosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.B1 | EndoQ | - |
Pyrococcus furiosus |
| 3.1.21.B1 | EndoQ | - |
Thermococcus kodakarensis |
| 3.1.21.B1 | PF1551 | locus name | Pyrococcus furiosus |
| 3.1.21.B1 | TK0887 | locus name | Thermococcus kodakarensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.B1 | 75 | - |
assay at | Pyrococcus furiosus |
| 3.1.21.B1 | 75 | - |
assay at | Thermococcus kodakarensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.B1 | 8 | - |
assay at | Pyrococcus furiosus |
| 3.1.21.B1 | 8 | - |
assay at | Thermococcus kodakarensis |
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Release 2023.1 (January 2023)
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