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Literature summary extracted from

  • Lai, S.M.; Lai, L.B.; Foster, M.P.; Gopalan, V.
    The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA (2014), Nucleic Acids Res., 42, 13328-13338.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.5 C71V the single-Cys substitutions are introduced into a Cys-less Pfu L7Ae template C71V (i.e. RNA-binding protein L7Ae, subunit of archaeal RNase P). The native C71, which is partly buried, is mutated to Val to preserve the native fold and hydrophobic core of the protein. The C71V parental reference is more active than the wild type enzyme Pyrococcus furiosus
3.1.26.5 K42C mutation does not affect activity. The single-Cys substitution is introduced into a Cys-less Pfu L7Ae template C71V (i.e. RNA-binding protein L7Ae, subunit of archaeal RNase P). The native C71, which is partly buried, is mutated to Val to preserve the native fold and hydrophobic core of the protein Pyrococcus furiosus
3.1.26.5 R46C mutation results in 28% decrease in activity. The single-Cys substitution is introduced into a Cys-less Pfu L7Ae template C71V (i.e. RNA-binding protein L7Ae, subunit of archaeal RNase P). The native C71, which is partly buried, is mutated to Val to preserve the native fold and hydrophobic core of the protein Pyrococcus furiosus
3.1.26.5 V95C mutation results in 6% decrease in activity. The single-Cys substitution is introduced into a Cys-less Pfu L7Ae template C71V (i.e. RNA-binding protein L7Ae, subunit of archaeal RNase P). The native C71, which is partly buried, is mutated to Val to preserve the native fold and hydrophobic core of the protein Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 pre-tRNATyr + H2O pre-tRNATyr from Escherichia coli Pyrococcus furiosus tRNATyr + 5'-oligoribonucleotide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.5 oligomer the RNA-binding protein L7Ae (UniProt: Q8U160) is a subunit of the archaeal RNase P ribonucleoprotein complex. The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.1.26.5 RNase P
-
 Pyrococcus furiosus
3.1.26.5 RPR
-
 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.5 8
-
 assay at Pyrococcus furiosus