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Literature summary extracted from

  • Kleingardner, J.G.; Bren, K.L.
    Comparing substrate specificity between cytochrome c maturation and cytochrome c heme lyase systems for cytochrome c biogenesis (2011), Metallomics, 3, 396-403.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.4.1.17 molecular biology system III, cytochrome c heme lyase, is an enzyme found in the mitochondria of many eukaryotes, which is used for heterologous expression of mitochondrial holocytochromes c Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.17 expression of the enzyme in Escherichia coli cytosplasm, coexpression with several mutant variants of holocytochrome c552 from Hydrogenobacter thermophilus, the latter are expressed in the periplasm, or several cytochrome c variants from Equus caballus Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.17 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.17 Apocytochrome c + heme Saccharomyces cerevisiae
-
 Holocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.17 Saccharomyces cerevisiae P06182
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.17 Apocytochrome c + heme
-
 Saccharomyces cerevisiae Holocytochrome c
-
 ?
4.4.1.17 Apocytochrome c + heme activity of the recombinant enzyme with several mutant variants of holocytochrome c552 from Hydrogenobacter thermophilus coexpressed in Escherichia coli in cytoplasm and periplasm, respectively, and enzyme activity with several cytocohrome variants from Equus caballus in Escherichia coli cells, heme attachment motifs, overview Saccharomyces cerevisiae Holocytochrome c
-
 ?
4.4.1.17 additional information the enzyme shares the ability of CCM, i.e. System I, the cytochrome c maturation system, found in many bacteria and commonly employed in the maturation of bacterial cytochromes c in Escherichia coli-based expression systems, to mature hemes c with extended heme attachment motifs, comparison of System I and cytochrome c heme lyase, i.e. System III, substrate specificities, overview Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.17 CCHL
-
 Saccharomyces cerevisiae
4.4.1.17 CYC3
-
 Saccharomyces cerevisiae
4.4.1.17 cytochrome c heme lyase
-
 Saccharomyces cerevisiae
4.4.1.17 System III
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
4.4.1.17 evolution at least five heme c maturation systems have been identified: Systems I, II, III, IV, and V. System I, the cytochrome c maturation (CCM) system, involves genes ccmA-H5 and is found in various bacteria and in plant mitochondria. System I matures cytochromes c in the periplasmic space of bacteria. System II, cytochrome c synthesis (CCS), consists of four modular components and occurs in various bacteria as well as the thylakoid membranes of plants and algae. System III, also called cytochrome c heme lyase (CCHL), is a single enzyme found in the mitochondria of fungi, vertebrates and invertebrates Saccharomyces cerevisiae
4.4.1.17 additional information construction endogenous holocytochrome c mutant variant with a CX3CH motif. The second X residue in the heme binding motif of cyt c1 (CXXCH) is important for its recognition as a substrate by the enzyme Saccharomyces cerevisiae