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Literature summary extracted from

  • Lahiri, S.; Banerjee, S.; Dutta, T.; Sengupta, S.; Dey, S.; Roy, R.; Sengupta, D.; Chattopadhyay, K.; Ghosh, A.K.
    Enzymatic and regulatory attributes of trehalose-6-phosphate phosphatase from Candida utilis and its role during thermal stress (2014), J. Cell. Physiol., 229, 1245-1255.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.12 CDTA strong inhibition Cyberlindnera jadinii
3.1.3.12 EDTA strong inhibition Cyberlindnera jadinii
3.1.3.12 EGTA strong inhibition Cyberlindnera jadinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.12 0.82
-
trehalose 6-phosphate native enzyme, at pH 8.5 and 30°C Cyberlindnera jadinii
3.1.3.12 0.9
-
trehalose 6-phosphate recombinant enzyme, at pH 8.5 and 30°C Cyberlindnera jadinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.12 Ca2+ has significant positive effect on enzyme activity Cyberlindnera jadinii
3.1.3.12 Mg2+ required, almost 10fold increase in activity at 2 mM Cyberlindnera jadinii
3.1.3.12 Mn2+ has significant positive effect on enzyme activity Cyberlindnera jadinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.12 82000
-
x * 82000, SDS-PAGE Cyberlindnera jadinii
3.1.3.12 82108
-
x * 82108, MALDI-TOF mass spectrometry Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.12 trehalose 6-phosphate + H2O Cyberlindnera jadinii
-
trehalose + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.12 Cyberlindnera jadinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.12 ammonium sulfate precipitation, Protein-Pak DEAE column chromatography and Biosuite 125 gel filtration Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.12 0.01033
-
cell lysate, at pH 8.5 and 30°C Cyberlindnera jadinii
3.1.3.12 0.4505
-
after 43.6fold purification, at pH 8.5 and 30°C Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.12 additional information no activity with sucrose-6-phosphate, fructose-6-phosphate, glucose-6-phosphate, fructose-6-phosphate, mannose-6-phosphate, 4-nitrophenylphosphate, ATP, and ADP Cyberlindnera jadinii ?
-
?
3.1.3.12 trehalose 6-phosphate + H2O
-
Cyberlindnera jadinii trehalose + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.12 ? x * 82000, SDS-PAGE Cyberlindnera jadinii
3.1.3.12 ? x * 82108, MALDI-TOF mass spectrometry Cyberlindnera jadinii

Synonyms

EC Number Synonyms Comment Organism
3.1.3.12 TPP
-
Cyberlindnera jadinii
3.1.3.12 trehalose-6-phosphate phosphatase
-
Cyberlindnera jadinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.12 30
-
-
Cyberlindnera jadinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.12 30 60 the enzyme is most stable up to 30°C. The enzyme is also able to show almost 50% activity at temperatures as high as 60°C Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.12 8.5
-
-
Cyberlindnera jadinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.12 4 12 the enzyme retains almost 60% activity in highly alkaline condition (pH 10.0) or moderately acidic condition (pH 6.0). Almost 40% activity still remains in pH as low as 4.0 or as high as 12.0 Cyberlindnera jadinii

Expression

EC Number Organism Comment Expression
3.1.3.12 Cyberlindnera jadinii almost 1.8fold increase in activity is observed when the concentration of S-adenosyl-L-methionine is increased from 0 to 2 mM up